煤化工反渗透浓水的高效降解菌株筛选、鉴定及应用研究

doi:10.11949/0438-1157.20210212

化工学报 ›› 2021, Vol. 72 ›› Issue (9): 4881-4891.DOI: 10.11949/0438-1157.20210212

煤化工反渗透浓水的高效降解菌株筛选、鉴定及应用研究

黄莉婷¹(),韩昫身²(),金艳³,马强⁴,于建国¹()

^1.华东理工大学资源过程工程教育部工程研究中心，上海 200237
^2.华东理工大学国家盐湖资源综合利用工程技术研究中心，上海 200237
^3.苏州聚智同创环保科技有限公司，江苏常熟 215513
^4.中国石油川庆钻探工程有限公司页岩气勘探开发项目经理部，四川成都 610051

收稿日期:2021-02-03 修回日期:2021-06-06 出版日期:2021-09-05 发布日期:2021-09-05
通讯作者: 韩昫身,于建国
作者简介:黄莉婷(1996—)，女，硕士研究生，hliting2020@163.com
基金资助:
上海市青年科技英才扬帆计划项目(20YF1409500);中央高校基本科研业务费专项资金(50321022017008)

Isolation, identification and application of highly efficient halotolerant strains for coal chemical reverse osmosis concentrate treatment

Liting HUANG¹(),Xushen HAN²(),Yan JIN³,Qiang MA⁴,Jianguo YU¹()

^1.Engineering Research Center of Resource Process Engineering, Ministry of Education, East China University of Science and Technology, Shanghai 200237, China
^2.National Engineering Research Center for Integrated Utilization of Salt Lake Resources, East China University of Science and Technology, Shanghai 200237, China
^3.Suzhou Juzhi Tongchuang Environmental Technology Co. , Ltd. , Changshu 215513, Jiangsu, China
^4.Shale Gas Exploration and Development Project Manager Department, CNPC Chuanqing Drilling Engineering Co. , Ltd. , Chengdu 610051, Sichuan, China

Received:2021-02-03 Revised:2021-06-06 Online:2021-09-05 Published:2021-09-05
Contact: Xushen HAN,Jianguo YU

摘要/Abstract

摘要：

一般煤化工废水经过多级氧化处理后，反渗透淡水回用、浓水经蒸发产生难处理的“危废”，有机物的存在对“危废”循环利用有显著制约作用。以煤化工反渗透浓水为底物（TOC为233.4 mg/L，TDS为50.9 g/L，BOD₅/COD仅为0.05），从不同菌源中筛选得到9株高效耐盐菌，经16S rDNA测序表明，这些菌株属于假单胞菌属、芽孢杆菌属及嗜盐单胞菌属。将9株耐盐菌配制成复合耐盐菌剂连续式运行处理实际废水，有机物去除率可达30%，为进一步提高去除率，经臭氧氧化预处理，有机物去除率可提高至40%，达到国内外较先进水平。根据气质联用分析，臭氧氧化预处理会破坏废水中环状物质的结构，提高复合耐盐菌剂对难降解有机物的去除效果。本研究为煤化工反渗透浓水中有机物的生物降解提供了可行性方案。

关键词: 废水, 降解, 污染, 煤化工废水, 反渗透浓水, 嗜（耐）盐菌, 复合耐盐菌剂, 臭氧氧化

Abstract:

Coal chemical industry plays an important role in national economy of China. Currently, coal chemical wastewater is usually treated by chemical oxidation and biochemical oxidation to remove the organic matter, followed by reverse osmosis (RO) to get the recycled fresh water. The concentrate is subsequently evaporated, while the generated mixture of salts and organic matter belongs to hazardous waste, which is costly to be treated and not environmentally friendly. With the increasing requirements of environmental protection, fractional crystallization has been developed to solve this problem. However, organic matter significantly inhibits salt crystallization. Consequently, there is considerable interest in removing the organic matter of coal chemical RO concentrate efficiently and economically. The cost of advanced oxidation treatment is higher, while it is hard to conduct microbiological treatment in such wastewater since it is weak in high salinity and concentrated toxic compounds. In the work reported here, coal chemical RO concentrate contained 50.9 g/L of TDS and 233.4 mg/L of TOC (total organic carbon) with BOD₅/COD of 0.05. Nine halotolerant bacteria strains were successfully isolated from different sludge and soil, which belonged to Pseudomonas sp., Bacillus sp., and Halomonas sp.by16S rDNA identification. Meanwhile, the research has studied physiological and biochemical characteristics of these nine strains. The results showed that the strains can grow well at 0—15% salinity. Ozone oxidation pretreatment and halotolerant bacteria preparation (mixture of nine strains) treatment was used to treat the wastewater. After one month's continuous operation, the TOC removal ratio reached 40%, reflecting an advanced level compared to the previous study. According to GC-MS (gas chromatography-mass spectrometer) analysis, ozone oxidation can destroy the cyclic structure of organic matter and improve the TOC removal ratio of subsequent halotolerant bacteria preparation. This work provided an effective approach to degrade coal chemical reverse osmosis concentrate by microbial method.

Key words: waste water, degradation, pollution, coal chemical wastewater, reverse osmosis concentrate, halophilic and halotolerant bacteria, halotolerant bacteria preparation, ozone oxidation

中图分类号:

X 172

黄莉婷, 韩昫身, 金艳, 马强, 于建国. 煤化工反渗透浓水的高效降解菌株筛选、鉴定及应用研究[J]. 化工学报, 2021, 72(9): 4881-4891.

Liting HUANG, Xushen HAN, Yan JIN, Qiang MA, Jianguo YU. Isolation, identification and application of highly efficient halotolerant strains for coal chemical reverse osmosis concentrate treatment[J]. CIESC Journal, 2021, 72(9): 4881-4891.

图/表 10

参考文献 45

46	任明, 孙淑英, 金艳, 等. 催化臭氧氧化法处理煤化工高盐废水[J]. 环境工程, 2018, 36(8): 54-59.
	Ren M, Sun S Y, Jin Y, et al. Treatment of high-salt wastewater from coal chemical industry by catalytic ozone oxidation[J]. Environmental Engineering, 2018, 36(8): 54-59.
47	Fang F, Han H J. Effect of catalytic ozonation coupling with activated carbon adsorption on organic compounds removal treating RO concentrate from coal gasification wastewater[J]. Ozone: Science & Engineering, 2018, 40(4): 275-283.
48	Ji Q H, Tabassum S, Hena S, et al. A review on the coal gasification wastewater treatment technologies: past, present and future outlook[J]. Journal of Cleaner Production, 2016, 126: 38-55.
1	任同伟, 俞彬, 阳春芳, 等. 煤化工高含盐废水资源化处理技术的工程应用研究[J]. 工业水处理, 2019, 39(2): 96-99.
	Ren T W, Yu B, Yang C F, et al. Research on the engineering application of the recycling treatment technology of high salinity wastewater in coal chemical industry[J]. Industrial Water Treatment, 2019, 39(2): 96-99.
2	杜松, 金文标, 刘宁, 等. 煤化工高含盐废水有机物的去除研究[J]. 煤炭科学技术, 2019, 47(12): 221-225.
	Du S, Jin W B, Liu N, et al. Study on removal of organic matter from high-salt wastewater in coal chemical industry[J]. Coal Science and Technology, 2019, 47(12): 221-225.
3	陈莉荣, 邬东, 谷振超, 等. 煤化工含盐废水的处理技术应用进展[J]. 工业水处理, 2019, 39(12): 12-18.
	Chen L R, Wu D, Gu Z C, et al. Technology application on salt-containing wastewater treatment in coal chemical industry[J]. Industrial Water Treatment, 2019, 39(12): 12-18.
4	赵婷婷, 王真, 郑雯倩. 探讨煤化工废水的处理技术及应用[J]. 中国资源综合利用, 2019, 37(5): 64-66.
	Zhao T T, Wang Z, Zheng W Q. Discussion on treatment technology and application of coal chemical wastewater[J]. China Resources Comprehensive Utilization, 2019, 37(5): 64-66.
5	曲风臣. 煤化工废水"零排放"技术要点及存在问题[J]. 化学工业, 2013, 31(Z1): 18-24.
	Qu F C. The key technologies and problems of wastewater zero discharge in coal chemical industry[J]. Chemical Industry, 2013, 31(Z1): 18-24.
6	Liu C, Chen X X, Zhang J, et al. Advanced treatment of bio-treated coal chemical wastewater by a novel combination of microbubble catalytic ozonation and biological process[J]. Separation and Purification Technology, 2018, 197: 295-301.
7	Zheng L X, Han X S, Han T, et al. Formulating a fully converged biorefining chain with zero wastewater generation by recycling stillage liquid to dry acid pretreatment operation[J]. Bioresource Technology, 2020, 318: 124077.
8	Bian C, Chen H, Song X F, et al. Metastable zone width and the primary nucleation kinetics for cooling crystallization of NaNO₃ from NaCl-NaNO₃-H₂O system[J]. Journal of Crystal Growth, 2019, 518: 5-13.
9	Bian C, Chen H, Song X F, et al. Effects of organic pollutants on the fractional crystallization of NaNO₃ from high-saline wastewater[J]. Journal of Crystal Growth, 2020, 540: 125656.
10	Liu J, Ou H S, Wei C H, et al. Novel multistep physical/chemical and biological integrated system for coking wastewater treatment: technical and economic feasibility[J]. Journal of Water Process Engineering, 2016, 10: 98-103.
11	Huang Y, Hou X L, Liu S T, et al. Correspondence analysis of bio-refractory compounds degradation and microbiological community distribution in anaerobic filter for coking wastewater treatment[J]. Chemical Engineering Journal, 2016, 304: 864-872.
12	Pradhan S, Fan L H, Roddick F A, et al. Impact of salinity on organic matter and nitrogen removal from a municipal wastewater RO concentrate using biologically activated carbon coupled with UV/H₂O₂[J]. Water Research, 2016, 94: 103-110.
13	Pradhan S, Fan L H, Roddick F A. Removing organic and nitrogen content from a highly saline municipal wastewater reverse osmosis concentrate by UV/H₂O₂-BAC treatment[J]. Chemosphere, 2015, 136: 198-203.
14	Jia S Y, Han Y X, Zhuang H F, et al. Simultaneous removal of organic matter and salt ions from coal gasification wastewater RO concentrate and microorganisms succession in a MBR[J]. Bioresource Technology, 2017, 241: 517-524.
15	Liu R K, Wang Q, Li M, et al. Advanced treatment of coal chemical reverse osmosis concentrate with three-stage MABR[J]. RSC Advances, 2020, 10(17): 10178-10187.
16	Lan M C, Li M, Liu J, et al. Coal chemical reverse osmosis concentrate treatment by membrane-aerated biofilm reactor system[J]. Bioresource Technology, 2018, 270: 120-128.
17	Liu X D, Wu S J, Zhang D J, et al. Simultaneous pyridine biodegradation and nitrogen removal in an aerobic granular system[J]. Journal of Environmental Sciences, 2018, 67: 318-329.
18	唐婧, 屈姗姗, 傅金祥, 等. 复合菌剂强化处理高盐废水脱氮效果[J]. 环境工程学报, 2015, 9(6): 2699-2705.
	Tang J, Qu S S, Fu J X, et al. Efficiency of saline wastewater denitrification by bioaugmentation with composite microbial inoculum[J]. Chinese Journal of Environmental Engineering, 2015, 9(6): 2699-2705.
19	Abou-Elela S I, Kamel M M, Fawzy M E. Biological treatment of saline wastewater using a salt-tolerant microorganism[J]. Desalination, 2010, 250(1): 1-5.
20	Zhuang X L, Han Z, Bai Z H, et al. Progress in decontamination by halophilic microorganisms in saline wastewater and soil[J]. Environmental Pollution, 2010, 158(5): 1119-1126.
21	金艳, 张永红, 宋兴福, 等. 一株降解页岩气采出水耐盐菌的分离鉴定与特性[J]. 华东理工大学学报(自然科学版), 2020, 46(6): 722-729.
	Jin Y, Zhang Y H, Song X F, et al. Identification and characteristics of a salt-tolerant bacteria for shale gas produced water treatment[J]. Journal of East China University of Science and Technology, 2020, 46(6): 722-729.
22	金艳, 张永红, 宋兴福, 等. 耐盐菌MBR系统处理页岩气采出水性能及膜污染特性[J]. 华东理工大学学报(自然科学版), 2020, 46(6): 730-736.
	Jin Y, Zhang Y H, Song X F, et al. Performance and membrane fouling of produced water from shale gas treated by MBR system with salt-tolerant bacteria[J]. Journal of East China University of Science and Technology, 2020, 46(6): 730-736.
23	Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10): 2731-2739.
24	Thompson J D, Higgins D G, Gibson T J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research, 1994, 22(22): 4673-4680.
25	孟凡旭, 吴敏, 张会斌, 等. 阿牙克库木湖嗜盐菌的分离及功能酶的筛选[J]. 浙江大学学报(理学版), 2006, 33(6): 671-675.
	Meng F X, Wu M, Zhang H B, et al. Isolation and enzyme screening of halophilesfrom Ayakekum Lake[J]. Journal of Zhejiang University (Science Edition), 2006, 33(6): 671-675.
26	王晓丽, 于建国. 一个甲烷氧化菌株的分离、鉴定及其特性研究[J]. 微生物学通报, 2008, 35(6): 934-938.
	Wang X L, Yu J G. Isolation, identification and characterization of a methanotrophic strain[J]. Microbiology, 2008, 35(6): 934-938.
27	张浩, 刘玉香, 呼婷婷, 等. 一株苯胺降解菌的分离及其降解特性[J]. 环境工程学报, 2015, 9(12): 6154-6160.
	Zhang H, Liu Y X, Hu T T, et al. Isolation and characterization of an aniline-degrading bacterium[J]. Chinese Journal of Environmental Engineering, 2015, 9(12): 6154-6160.
28	高廷耀, 顾国维, 周琪. 水污染控制工程[M]. 北京: 高等教育出版社, 2007.
	Gao T Y, Gu G W, Zhou Q. Water Pollution Control Engineering [M]. Beijing: Higher Education Press, 2007.
29	Ahmadi M, Ahmadmoazzam M, Saeedi R, et al. Biological treatment of a saline and recalcitrant petrochemical wastewater by using a newly isolated halo-tolerant bacterial consortium in MBBR[J]. Desalination and Water Treatment, 2019, 167: 84-95.
30	Qu J H, Chen X L, Zhou J, et al. Treatment of real sodium saccharin wastewater using multistage contact oxidation reactor and microbial community analysis[J]. Bioresource Technology, 2019, 289: 121714.
31	Yan Y X, Yang J, Zhu Z Y, et al. Enhancing performance evaluation and microbial community analysis of the biofilter for toluene removal by adding polyethylene glycol-600 into the nutrient solution[J]. Bioresource Technology, 2021, 330: 124954.
32	Wang Q, Wu X G, Jiang L H, et al. Effective degradation of di-n-butyl phthalate by reusable, magnetic Fe₃O₄ nanoparticle-immobilized Pseudomonas sp. W1 and its application in simulation[J]. Chemosphere, 2020, 250: 126339.
33	Wang J L, Zhou J, Wang Y M, et al. Efficient nitrogen removal in a modified sequencing batch biofilm reactor treating hypersaline mustard tuber wastewater: the potential multiple pathways and key microorganisms[J]. Water Research, 2020, 177: 115734.
34	Zerva I, Remmas N, Melidis P, et al. Biotreatment efficiency, hydrolytic potential and bacterial community dynamics in an immobilized cell bioreactor treating caper processing wastewater under highly saline conditions[J]. Bioresource Technology, 2021, 325: 124694.
35	Haddadi A, Shavandi M. Biodegradation of phenol in hypersaline conditions by Halomonas sp strain PH₂-2 isolated from saline soil[J]. International Biodeterioration & Biodegradation, 2013, 85: 29-34.
36	Piubeli F, Grossman M J, Fantinatti-Garboggini F, et al. Enhanced reduction of COD and aromatics in petroleum-produced water using indigenous microorganisms and nutrient addition[J]. International Biodeterioration & Biodegradation, 2012, 68: 78-84.
37	Fimlaid K A, Shen A. Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes[J]. Current Opinion in Microbiology, 2015, 24: 88-95.
38	Khanpour-Alikelayeh E, Partovinia A, Talebi A, et al. Investigation of Bacilluslicheniformis in the biodegradation of Iranian heavy crude oil: a two-stage sequential approach containing factor-screening and optimization[J]. Ecotoxicology and Environmental Safety, 2020, 205: 111103.
39	Koops H P, Pommerening-Röser A. Distribution and ecophysiology of the nitrifying bacteria emphasizing cultured species[J]. FEMS Microbiology Ecology, 2001, 37(1): 1-9.
40	Santorelli M, Maurelli L, Pocsfalvi G, et al. Isolation and characterisation of a novel alpha-amylase from the extreme haloarchaeon Haloterrigena turkmenica[J]. International Journal of Biological Macromolecules, 2016, 92: 174-184.
41	Kargi F, Dinçer A R. Saline wastewater treatment by halophile-supplemented activated sludge culture in an aerated rotating biodisc contactor[J]. Enzyme and Microbial Technology, 1998, 22(6): 427-433.
42	Hou M, Li W, Li H, et al. Performance and bacterial characteristics of aerobic granular sludge in response to alternating salinity[J]. International Biodeterioration & Biodegradation, 2019, 142: 211-217.
43	Wang D X, Han Y X, Han H J, et al. Enhanced treatment of Fischer-Tropsch wastewater using up-flow anaerobic sludge blanket system coupled with micro-electrolysis cell: a pilot scale study[J]. Bioresource Technology, 2017, 238: 333-342.
44	俞汉青, 郑煜铭, 顾国维, 等. 活性污泥对四种非极性有机物的吸附[J]. 环境科学学报, 2003, 23(4): 546-548.
	Yu H Q, Zheng Y M, Gu G W, et al. Sorption of four non-poplar organic compounds by activated sludge[J]. Acta Scientiae Circumstantiae, 2003, 23(4): 546-548.
45	Álvarez P M, Beltrán F J, Pocostales J P, et al. Preparation and structural characterization of Co/Al₂O₃ catalysts for the ozonation of pyruvic acid[J]. Applied Catalysis B: Environmental, 2007, 72(3/4): 322-330.

菌株编号	相似菌株	相似度/%	序列号
L-141	Pseudomonas sp. HC2-4	100.00	JF312947.1
L-142	Pseudomonas sp. strain P0u25	99.92	MK737101.1
L-143	Bacillus sp. (in: Bacteria) strain CY1TSA7	99.79	MH974115.1
L-144	Halomonas alkaliphila X3	100.00	CP024811.1
L-145	Bacillus altitudinis strain HQ-51-Ba	100.00	CP040747.1
L-146	Bacillus altitudinis strain HQ-51-Ba	99.85	CP040747.1
L-147	Bacillus sp. (in: Bacteria) strain CY1TSA7	100.00	MH974115.1
L-275	Bacillus flexus strain HDB-2	99.72	MK178593.1
L-276	Bacillus altitudinis strain NPB34b	100.00	MT598007.1

菌株编号	相似菌株	相似度/%	序列号
L-141	Pseudomonas sp. HC2-4	100.00	JF312947.1
L-142	Pseudomonas sp. strain P0u25	99.92	MK737101.1
L-143	Bacillus sp. (in: Bacteria) strain CY1TSA7	99.79	MH974115.1
L-144	Halomonas alkaliphila X3	100.00	CP024811.1
L-145	Bacillus altitudinis strain HQ-51-Ba	100.00	CP040747.1
L-146	Bacillus altitudinis strain HQ-51-Ba	99.85	CP040747.1
L-147	Bacillus sp. (in: Bacteria) strain CY1TSA7	100.00	MH974115.1
L-275	Bacillus flexus strain HDB-2	99.72	MK178593.1
L-276	Bacillus altitudinis strain NPB34b	100.00	MT598007.1

菌株编号	革兰染色	氧化酶	触酶	淀粉酶	脲酶	吲哚	酪素水解	产H₂S	明胶水解	好氧/厌氧
L-141	-	-	-	+	+	-	+	-	-	+
L-142	+	-	-	-	+	-	+	-	-	+
L-143	+	-	-	-	+	-	+	-	-	+
L-144	+	-	-	-	+	-	+	-	-	+
L-145	+	-	-	-	+	-	+	-	-	+
L-146	+	-	-	-	+	-	+	-	-	+
L-147	+	-	-	-	+	-	+	-	-	+
L-275	+	-	-	-	+	-	+	-	-	+
L-276	+	-	-	+	+	-	+	-	-	+

菌株编号	革兰染色	氧化酶	触酶	淀粉酶	脲酶	吲哚	酪素水解	产H₂S	明胶水解	好氧/厌氧
L-141	-	-	-	+	+	-	+	-	-	+
L-142	+	-	-	-	+	-	+	-	-	+
L-143	+	-	-	-	+	-	+	-	-	+
L-144	+	-	-	-	+	-	+	-	-	+
L-145	+	-	-	-	+	-	+	-	-	+
L-146	+	-	-	-	+	-	+	-	-	+
L-147	+	-	-	-	+	-	+	-	-	+
L-275	+	-	-	-	+	-	+	-	-	+
L-276	+	-	-	+	+	-	+	-	-	+

煤化工反渗透浓水		复合耐盐菌剂法产水		臭氧预处理产水		臭氧预处理和复合耐盐菌剂法联合工艺产水
有机物成分	占比/%	有机物成分	占比/%	有机物成分	占比/%	有机物成分	占比/%
乙二醇单丁醚	2.8	四氧杂环己烷	4.1	乙醇	8.5	5-乙酰氧基-6(1,2-环氧丙基)-5,6二氢吡喃-2-酮	4.5
乙二醇单丁醚	2.8	四氧杂环己烷	4.1	四氧杂环己烷	0.7	5-乙酰氧基-6(1,2-环氧丙基)-5,6二氢吡喃-2-酮	4.5
四氧杂环己烷	1.9	1-亚硝基-2-哌啶甲酸	3.1	二氯异乙醚	40.9	1-甲基-环戊烷羧酸	4.9
1-亚硝基-2-哌啶甲酸	3.9	环己胺	2.5	3-己烯-2,5-二醇	5.5	3-乙基-2-甲基-1-戊烯	5.9
硫代氨基甲酸,N-正环己基S-(2,5-二羟基苯基)	2.7	5-甲基-4-乙烯-3-酮	4.5	2,3-双环呋喃	4.3	2,3-双环呋喃	5.7
2,2-甲基-3-乙烯	6.5	2,3-双环呋喃	4.9	3-乙基-4-甲基-2-戊烯	3.9	2,4-二叔丁基苯酚	9.9
2,3-双环呋喃	9.3	乙基环己烷	1.8	3-乙基-2-戊烯	1.4
5,5-二甲基-3-环己烯-1-醇	23.1	6-exo-vinyl-5-endo-norbornenol	25.9	氯丙醇	0.6
对二氮杂环	2.4	2,6-环辛二烯-1-醇	2.1	二十烷	0.4
2,4-二叔丁基苯酚	2.8			2,4-二叔丁基苯酚	0.7
2,4-二叔丁基苯酚	2.8			二十六烷	0.8

煤化工反渗透浓水的高效降解菌株筛选、鉴定及应用研究

Isolation, identification and application of highly efficient halotolerant strains for coal chemical reverse osmosis concentrate treatment

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