关键词:

多通道流动电泳, 计算流体力学, 模拟

Abstract: This paper presents computational fluid dynamics (CFD) simulation of fluid flow and protein migration in multichannel flow electrophoresis (MFE). The simulation shows that natural convection created by Joule heat occurs along the direction of electric field and influences the apparent electrokinetic migration of protein. Furthermore, the factors affecting natural convection are examined. The scaling up of the MFE apparatus is attempted by extending the dimension along the flow direction. In this case, an identical fluid flow status could be obtained for MFE apparatus of different scales, as suggested by CFD simulation. The validity of this scaling up strategy is demonstrated by fairly good agreement between the theoretical prediction and the experimental results obtained in MFE apparatus of different scales.

Key words:

多通道流动电泳, 计算流体力学, 模拟