关键词: 扩张床吸附, 纤维素, 钛白粉, 离子交换, 蛋白质

Abstract: Expanded bed adsorption (EBA) has been widely used in the industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-TiO2 composite matrix was prepared with the method of water-in-oil suspension thermal regeneration. After activating by epichlorohydrin and coupling with diethylamine,the matrix was obtained to function as an anion exchanger. The experiments in expanded bed indicated that the adsorbent exhibited a stable expansion,approximate plug flow and the comparable column efficiency with the case in packed bed, providing a bovine serum albumin(BSA) adsorption capacity of 42.6 mg•ml^-1 adsorbent at 10% breakthrough.

Key words: 扩张床吸附, 纤维素, 钛白粉, 离子交换, 蛋白质