化工学报 ›› 2011, Vol. 62 ›› Issue (S1): 151-156.

• 分离工程 • 上一篇    下一篇

基于电化学技术检测微生物吸附

曹生现,欧佳,张艳辉,杨善让,徐志明   

  1. 东北电力大学自动化学院;东北电力大学化学工程学院;东北电力大学能源与动力工程学院,吉林省 吉林市 132012
  • 出版日期:2011-07-03 发布日期:2011-07-03

  • Online:2011-07-03 Published:2011-07-03

摘要:

为研究微生物在固体表面吸附的过程,探索微生物黏膜的形成机理,采用电化学交流阻抗法对铜电极表面黏液形成菌吸附形成的生物黏膜进行表征,实验结果表明:由实验数据拟合出的等效电路与根据理论分析而设计出的等效电路基本吻合,通过测量生物膜电容计算出的生物黏膜厚度为1.8 μm,与用SEM观测显示的黏膜厚度基本一致,由此说明了用交流阻抗表征生物膜的可行性和微生物在固体表面吸附传质成膜过程分析的正确性,该方法为探索微生物黏膜的形成机理提供了有力的检测手段,并对静态下实时监测生物黏膜的形成具有重要意义。

关键词: 微生物黏膜, 交流阻抗, 吸附, 黏液形成菌

Abstract:

Electrochemical impedance spectroscopy(EIS) is used to characterize the biofilm formation of Slime-forming bacteria on copper electrode surface for studying microbial adsorption on solid surface and exploring the microbial formation mechanism.The experimental results show that the equivalent circuit by experimental data fitting is basically consistent with the equivalent circuit based on theoretical analysis.The biofilm membrane thickness of 1.8 μm which was calculated by measuring the membrane capacitance is consistent with SEM observations.So, this research illustrates the feasibility of characterization of biofilms by using AC impedance and the correctness of microbial mass on the solid surface in the process of adsorption and the biofilm formation.This method is a powerful detection means for exploring the formation mechanism of biofilm and a great significance to real-time monitoring of the biofilm formation under the static state.

Key words: 微生物黏膜, 交流阻抗, 吸附, 黏液形成菌