关键词: 有机相, 电解质, 脂肪酶, 对映体选择性, 催化活力

Abstract: Lipase was an important biological catalyst，which was widely applied in many areas，how to improve its catalytic performance in non-natural environment has become a research hot spot gradually. Studies showed that the specific activity and enantioselectivity of lipase could be improved remarkably by adding some electrolytes into reaction system. However，relevant researches mainly focused on the role of single electrolyte，report on the types of electrolytes involved was much less，and further systematic research has not been reported yet. By utilizing Pseudomonas cepacia lipase （PcL） as the catalyst，the asymmetric transesterification resolution of 4-methylhept-4-en-1-yn-3-ol was conducted with the precursor of chiral chrysanthemum ester pesticides in hydrophobic organic media，the influences of 8 types，including halide，nitrate，sulfate，phosphate et al，35 kinds of electrolytes and their different combinations on PcL's catalytic performance were investigated systematically. The results indicate that the specific activity of PcL is 23 times higher than that of native lipase with synergistic effect of NaF，Na2HPO4 and NaH2PO4. Further study shows that the improvement of enantioselectivity has been achieved from 11 to 21，due to the different degree of activation of enantiomers by PcL.

Key words: organic media, electrolytes, lipase, enantioselectivity, catalytic activity