生物3D打印蓖麻油基水性聚氨酯涂层固定化碳酸酐酶

doi:10.11949/j.issn.0438-1157.20180054

化工学报 ›› 2018, Vol. 69 ›› Issue (8): 3577-3584.DOI: 10.11949/j.issn.0438-1157.20180054

生物3D打印蓖麻油基水性聚氨酯涂层固定化碳酸酐酶

崔建东^1,2, 崔兆惠^1,2, 苏志国², 郑春杨³, 马光辉², 张松平²

1 河北科技大学生物科学与工程学院, 河北石家庄 050018;
2 中国科学院过程工程研究所生化工程国家重点实验室, 北京 100190;
3 北京奥格诺生物科技有限公司, 北京 100192

收稿日期:2018-01-15 修回日期:2018-04-02 出版日期:2018-08-05 发布日期:2018-08-05
通讯作者: 张松平
基金资助:
国家自然科学基金项目（21676276，21676069，91534126）。

Bioactive coating prepared by bio-3D printing of castor oil-based waterborne polyurethane mixed with carbonic anhydrase

CUI Jiandong^1,2, CUI Zhaohui^1,2, SU Zhiguo², ZHENG Chunyang³, MA Guanghui², ZHANG Songping²

1 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, Hebei, China;
2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
3 Beijing Organoid Bio-Tech Co., Ltd., Beijing 100192, China

Received:2018-01-15 Revised:2018-04-02 Online:2018-08-05 Published:2018-08-05
Supported by:
supported by the National Natural Science Foundation of China (21676276, 21676069, 91534126).

摘要/Abstract

摘要：

以蓖麻油基阴离子水性聚氨酯为载体，采用生物3D打印技术制备含碳酸酐酶的生物活性聚氨酯涂层，并与传统涂覆法制备的含酶涂层进行对比。通过动态光散射、红外、扫描电镜-X射线能谱、热重、接触角等各种手段对涂层中酶与水性聚氨酯之间的相互作用进行了分析表征。结果表明，在形成生物活性涂层过程中，碳酸酐酶是通过与聚氨酯链段上阴离子基团的静电吸引被固定在聚氨酯涂层中。催化活性结果表明，与传统涂覆法相比，涂层的酶活回收率为50.51%，比传统涂覆法提高了4倍。这可能是由于生物3D打印技术制备的含酶涂层更薄、更均匀，表面更平滑。

关键词: 生物3D打印, 蓖麻油基水性聚氨酯, 涂层, 酶, 固定化, 生物催化

Abstract:

Castor oil-based anionic waterborne polyurethane (WPU) mixed with carbonic anhydrase (CA) was used to prepare bioactive coatings by bio-3D printing technique and regular coating methods. Interactions between CA and WPU were studied by dynamic light scattering, IR, SEM-EDC, TGA, and contact angle analyses. The results showed that the CA could attach tightly to WPU nanoparticles through the electrostatic interactions with anionic groups on the polyurethane. The activity recovery of bioactive coatings prepared by bio-3D printing technology was 4-fold higher than that of the conventional coating method, reaching 50.51%. The higher activity recovery could be attributed to the thin layer, smooth surface, and uniform distribution of CA in the 3D printed coating.

Key words: bio-3D printing, castor oil based waterborne polyurethane, coating, enzyme, immobilization, biocatalysis

中图分类号:

O643.3

崔建东, 崔兆惠, 苏志国, 郑春杨, 马光辉, 张松平. 生物3D打印蓖麻油基水性聚氨酯涂层固定化碳酸酐酶[J]. 化工学报, 2018, 69(8): 3577-3584.

CUI Jiandong, CUI Zhaohui, SU Zhiguo, ZHENG Chunyang, MA Guanghui, ZHANG Songping. Bioactive coating prepared by bio-3D printing of castor oil-based waterborne polyurethane mixed with carbonic anhydrase[J]. CIESC Journal, 2018, 69(8): 3577-3584.

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生物3D打印蓖麻油基水性聚氨酯涂层固定化碳酸酐酶

Bioactive coating prepared by bio-3D printing of castor oil-based waterborne polyurethane mixed with carbonic anhydrase

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