图1 用于线粒体靶向光疗的血清白蛋白-铜酞菁(LGS-CuPc-BSA)纳米粒子的制备及光疗过程

Fig.1 Schematic illustration of preparation of LGS-CuPc-BSA NPs for mitochondrial targeted phototherapy

图2 LGS-CuPc 和LGS-CuPc-BSA NPs的吸收光谱(a)和LGS-CuPc-BSA NPs的透射电镜图(b)

Fig.2 UV–vis absorption spectra of LGS-CuPc and LGS-CuPc-BSA NPs (a) and TEM image of LGS-CuPc-BSA NPs (b)

图3 在DMF中DPBF检测MB(a)、LGS-CuPc(b)、LGS-CuPc-BSA NPs(c)体系ROS的产生；MB、LGS-CuPc和LGS-CuPc-BSA NPs的时间与吸收的线性关系(d)

Fig.3 Singlet-oxygen generation of MB(a), LGS-CuPc(b), LGS-CuPc-BSA NPs (c) in DMF with DPBF, and the linear relationship between time and absorption of MB, LGS- CuPc, and LGS-CuPc-BSA NPs (d)

图4 光照(671 nm，800 mW·cm-2)不同浓度的LGS-CuPc(a)和LGS-CuPc-BSA NPs(b)的光热曲线温度变化，LGS-CuPc-BSA NPs五个循环的温度变化(每个循环20 min) (c)和τ值的计算曲线(d)

Fig.4 Photothermal heating curves for the change of temperature with time in different concentrations (20, 30, and 40 μmol·L-1) of LGS-CuPc (a), LGS-CuPc-BSA NPs irradiated by 671 nm laser (800 mW·cm-2) (b). The temperature variations of LGS-CuPc-BSA NPs for 5 cycles (20 min per cycle) (c). Calculation of τ value (d)

图5 在671 nm(800 mW·cm-2)激光照射10 min下经不同浓度的LGS-CuPc-BSA NPs(a)和LGS-CuPc(b)处理的细胞活性

Fig.5 Cell viabilities after treatments with different concentrations of LGS-CuPc-BSA NPs (a) and LGS-CuPc (b) with and without 671 nm (800 mW·cm-2) laser irradiation for 10 min

图6 经LGS-CuPc-BSA NPs和LGS-CuPc孵育的AM，PI双染共聚焦成像

Fig.6 Confocal images of co-stained MCF-7 cells with AM and PI in the presence of LGS-CuPc-BSA NPs and LGS-CuPc

图7 在MCF-7细胞中ROS的产生

Fig.7 ROS generation in MCF-7 cells

图8 Hoechst33342和LGS-CuPc-BSA NPs在MCF-7细胞中的荧光共聚焦成像

Fig.8 Confocal fluorescence images of Hoechst33342 and LGS-CuPc-BSA NPs in MCF-7 cells

图9 LGS-CuPc-BSA NPs与Mito-Tracker Green共定位成像

Fig.9 Co-localization imaging of LGS-CuPc-BSA NPs and Mito-Tracker Green in MCF-7 cells

图A1 LGS-CuPc, C.I.Direct Blue86的结构

Fig.A1 Structure of LGS-CuPc, C.I.Direct Blue86

图A2 BSA的透射电镜图

Fig.A2 TEM image of BSA

图A3 LGS-CuPc-BSA NPs在 PBS中的粒径分布

Fig.A3 Size distributions of LGS-CuPc-BSA NPs in PBS

图A4 LGS-CuPc-BSA NPs在PBS中的ζ电位

Fig.A4 The ζ potentials of LGS-CuPc-BSA NPs in aqueous solution

图A5 LGS-CuPc-BSA NPs在 PBS中的粒径和PDI

Fig.A5 Size and PDI of LGS-CuPc-BSA NPs in PBS

图A6 不同浓度的LGS-CuPc的紫外可见吸收光谱

Fig.A6 UV-vis absorption spectra of LGS-CuPc at different concentration

图A7 LGS-CuPc的浓度与671 nm吸收强度之间的线性关系

Fig.A7 The linear relationship between LGS-CuPc concentration and absorption intensity at 671 nm

图A8 体内荧光成像

Fig.A8 In vivo NIR fluorescencent images