化工学报 ›› 2021, Vol. 72 ›› Issue (12): 6298-6310.DOI: 10.11949/0438-1157.20211340

• 生物化学工程与技术 • 上一篇    下一篇

ARTP诱变钝顶螺旋藻突变体比较组学研究

苏楠1(),吴亦楠1(),陈韵亿1,金丽华2,张翀1,Aikawa Shimpei3,Hasunuma Tomohisa3,Kondo Akihiko3,邢新会1,4,5()   

  1. 1.清华大学化学工程系生物化工研究所,工业生物催化教育部重点实验室,合成与系统生物学中心,北京 100084
    2.北京电子科技职业技术学院生物技术系,北京 100029
    3.神户大学工程研究生院,化学科学与工程系,日本 神户 657-8501
    4.清华大学深圳国际研究生院生物医药与健康工程研究院,广东 深圳 518055
    5.深圳湾实验室医药健康技术与工程研究所,广东 深圳 518055
  • 收稿日期:2021-09-16 修回日期:2021-11-23 出版日期:2021-12-05 发布日期:2021-12-22
  • 通讯作者: 邢新会
  • 作者简介:苏楠(1983—),男,博士,sunan12345@126.com|吴亦楠(1992—),男,博士,wuyinan1992@qq.com
  • 基金资助:
    国家重点研发计划项目(2016YFD0102106);国家自然科学基金委国家重大科研仪器设备研制专项(21627812)

Comparative omics study of Spirulinaplatensis mutants based on ARTP mutagenesis breeding system

Nan SU1(),Yinan WU1(),Yinyee TAN1,Lihua JIN2,Chong ZHANG1,Aikawa SHIMPEI3,Hasunuma TOMOHISA3,Kondo AKIHIKO3,Xinhui XING1,4,5()   

  1. 1.Institute of Biochemical Engineering, Department of Chemical Engineering, Key Laboratory for Industrial Biocatalysis, Ministry of Education, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
    2.Department of Biotechnology, Beijing Electronic Science and Technology Vocational College, Beijing 100029, China
    3.Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
    4.Institute of Biomedicine and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, Guangdong, China
    5.Medical and Health Technology and Engineering Institute, Shenzhen Bay Laboratory, Shenzhen 518055, Guangdong, China
  • Received:2021-09-16 Revised:2021-11-23 Online:2021-12-05 Published:2021-12-22
  • Contact: Xinhui XING

摘要:

微藻作为地球上重要的生物资源,为水圈提供了大量的初级代谢产物,是合成生物学和生物制造研究和应用的重要底盘微生物。其中,钝顶螺旋藻(Spirulina platensis)具有多糖含量高、营养价值高、培养工艺成熟、应用范围广等特点,其诱变育种及比较组学研究可为微藻细胞工厂系统改造技术发展提供重要依据。本课题组前期通过常压室温等离子体(atmospheric and room temperature plasmas,ARTP)诱变方法获得了三株钝顶螺旋藻突变体。本研究在连续光照培养条件下,对三株突变体的重要生理特征进行了表征,发现突变株具有高絮凝表型,且重要化合物含量也与野生型具有一定的差异。进一步,本研究通过对其主要代谢产物的代谢组学分析和全基因组测序,对突变表型产生的机理进行了初步解析。

关键词: 钝顶螺旋藻, 絮凝, ARTP诱变, 代谢组学, 基因组比较组学, 生物能源, 生物工程, 生物技术

Abstract:

As an important biological resource on the earth, microalgae provides a large number of primary metabolites for the hydrosphere, and is an important chassis microorganism for the research and application of synthetic biology and biological manufacturing. Spirulina platensis is one of the cyanobacteria that has advantages of high polysaccharide content, high nutritional value, mature culture technology and wide application. The mutagenesis breeding and comparative omics studies can provide important basis for the development of microalgae cell-factory system modification. With atmospheric and room temperature plasmas (ARTP) mutagenesis method, we obtained three S. platensis mutants. By continuous light culture in this study, we characterized the mutants' important physiological characteristics. It was found that the mutants have much higher flocculation intensity than wild strain and differences in important metabolites content. Furthermore, through the mutants' metabolomic analysis and whole genome sequencing, we preliminarily analyzed the mechanism related to mutant phenotype.

Key words: Spirulina platensis, flocculation, ARTP mutagenesis, metabolomics, comparative genomics analysis, bioenergy, biological engineering, biotechnology

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