包埋菌启动厌氧氨氧化反应器及其动力学性能

doi:10.11949/j.issn.0438-1157.20141683

化工学报 ›› 2015, Vol. 66 ›› Issue (4): 1459-1466.DOI: 10.11949/j.issn.0438-1157.20141683

包埋菌启动厌氧氨氧化反应器及其动力学性能

陈光辉, 李军, 邓海亮, 张彦灼, 赵白航, 郑照明

北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124

收稿日期:2014-11-12 修回日期:2015-01-06 出版日期:2015-04-05 发布日期:2015-04-05
通讯作者: 李军
作者简介:陈光辉(1986-),男,博士研究生。
基金资助:
国家自然科学基金项目(51308010);国家水体污染控制与治理科技重大专项(2014ZX07201-011);北京市自然科学基金项目(8122005);北京市教委面上项目(KM201210005028)。

Performance and kinetic characteristics of immobilized granules on start-up of Anammox bioreactor

CHEN Guanghui, LI Jun, DENG Hailiang, ZHANG Yanzhuo, ZHAO Baihang, ZHENG Zhaoming

Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China

Received:2014-11-12 Revised:2015-01-06 Online:2015-04-05 Published:2015-04-05
Supported by:
supported by the National Natural Science Foundation of China (51308010), the National Critical Project for Science and Technology on Water Pollution Prevention and Control of China (2014ZX07201-011), the Natural Science Fund Project of Beijing (8122005) and Beijing Education Commission Funded Project (KM201210005028).

摘要/Abstract

摘要：

以厌氧氨氧化菌包埋颗粒代替颗粒污泥作为流加菌启动厌氧氨氧化反应器,并采用批次试验研究了包埋颗粒的动力学特性。结果表明,以包埋颗粒作为流加菌,在49 d内成功实现了厌氧氨氧化反应器的加速启动,反应器对NH⁺₄-N和NO^-₂-N的去除率分别为80.7%和83.1%,总氮去除负荷为0.505 kg·m^-3·d^-1。动力学研究表明,包埋颗粒对氨氮和亚硝氮的半速率常数分别为1.57 mmol·L^-1和1.505 mmol·L^-1。包埋颗粒对氨和亚硝酸的抑制常数分别为724.2 mmol·L^-1和66.65 mmol·L^-1。厌氧氨氧化菌包埋颗粒具有优良的动力学特性,作为流加菌效果显著,对新型厌氧氨氧化菌种流加技术的发展具有积极的意义。

关键词: 厌氧氨氧化, 包埋菌, 固定化, 启动, 动力学

Abstract:

Immobilized Anammox granules were used as sequential biocatalyst addition to substitute particulate sludge for starting up Anammox reactor, and the kinetic characteristics of the immobilized granules were investigated through batch tests. The reactor could be successfully started up in 49 d with immobilized Anammox granules as sequential biocatalyst addition, and the removal rates of NH⁺₄-N and NO^-₂-N were 80.7% and 83.1%, respectively. Kinetics study showed that the half saturation constants for ammonium and nitrite of the immobilized granules were 1.57 mmol·L^-1and 1.505 mmol·L^-1, the half inhibition constants of ammonium and nitrite were 724.2 mmol·L^-1and 66.65 mmol·L^-1, respectively. The immobilized granules were easier to gain substrate in adverse environment and could suffer higher substrate concentration. The better kinetic performance of the immobilized Anammox granules contributed to the successful biomass supplementation performance, which was helpful for the novel biomass supplementation process for enhancement of Anammox performance.

Key words: anaerobic ammonium oxidation, immobilized granules, immobilization, start-up, kinetics

中图分类号:

X703.1

陈光辉, 李军, 邓海亮, 张彦灼, 赵白航, 郑照明. 包埋菌启动厌氧氨氧化反应器及其动力学性能[J]. 化工学报, 2015, 66(4): 1459-1466.

CHEN Guanghui, LI Jun, DENG Hailiang, ZHANG Yanzhuo, ZHAO Baihang, ZHENG Zhaoming. Performance and kinetic characteristics of immobilized granules on start-up of Anammox bioreactor[J]. CIESC Journal, 2015, 66(4): 1459-1466.

参考文献 36

[1]	van de Graaf A A, de Bruijn P, Robertson L A, Jetten M S, Kuenen J G. Metabolic pathway of anaerobic ammonium oxidation on the basis of 15N studies in a fluidized bed reactor [J]. Microbiology, 1997, 143: 2415-2421
[2]	Tang Chongjian(唐崇俭), Zheng Ping(郑平), Chen Tingting(陈婷婷). Inoculum, start-up and performance of Anammox process [J]. CIESC Journal(化工学报), 2010, 61: 2510-2516
[3]	Jetten M S M, Wagner M, Fuerst J, van Loosdrecht M, Kuenen G, Strous M. Microbiology and application of the anaerobic ammonium oxidation (‘anammox') process [J]. Current Opinion in Biotechnology, 2001, 12: 283-288
[4]	Cong Yan(丛岩), Huang Xiaoli(黄晓丽), Wang Xiaolong(王小龙), Gao Dawen(高大文). Faster formation of Anammox granular sludge [J]. CIESC Journal(化工学报), 2014, 65: 664-671
[5]	Hendrickx T L, Kampman C, Zeeman G, Temmink H, Hu Z, Kartal B, Buisman C J. High specific activity for anammox bacteria enriched from activated sludge at 10℃ [J]. Bioresource Technology, 2014, 163: 214-221
[6]	Tsushima I, Ogasawara Y, Kindaichi T, Satoh H, Okabe S. Development of high-rate anaerobic ammonium-oxidizing (anammox) biofilm reactors [J]. Water Research, 2007, 41: 1623-1634
[7]	Jin R C, Yang G F, Zhang Q Q, Ma C, Yu J J, Xing B S. The effect of sulfide inhibition on the ANAMMOX process [J]. Water Research, 2013, 47: 1459-1469
[8]	Tang C J, Zheng P, Chen T T, Zhang J Q, Mahmood Q, Ding S, Chen X G, Chen J W, Wu D T. Enhanced nitrogen removal from pharmaceutical wastewater using SBA-ANAMMOX process [J]. Water Research, 2011, 45: 201-210
[9]	Gilbert E M, Agrawal S, Karst S M, Horn H, Nielsen P H, Lackner S. Low temperature partial nitritation/anammox in a moving bed biofilm reactor treating low strength wastewater [J]. Environmental Science & Technology, 2014
[10]	Dosta J, Fernandez I, Vazquez Padin J, Mosquera Corral A, Campos J, Mata Alvarez J, Mendez R. Short- and long-term effects of temperature on the Anammox process [J]. Journal of Hazardous Materials, 2008, 154: 688-693
[11]	van der Star W R, Abma W R, Blommers D, Mulder J W, Tokutomi T, Strous M, Picioreanu C, van Loosdrecht M. Startup of reactors for anoxic ammonium oxidation: experiences from the first full-scale anammox reactor in Rotterdam [J]. Water Research, 2007, 41: 4149-4163
[12]	Wett B. Solved upscaling problems for implementing deammonification of rejection water [J]. Water Science & Technology, 2006, 53: 121-128
[13]	López H, Puig S, Ganigué R, Ruscalleda M, Balaguer M D, Colprim J. Start-up and enrichment of a granular anammox SBR to treat high nitrogen load wastewaters [J]. Journal of Chemical Technology and Biotechnology, 2008, 83: 233-241
[14]	Dapena-Mora A, Campos J L, Mosquera-Corral A, Jetten M, Méndez R. Stability of the ANAMMOX process in a gas-lift reactor and a SBR [J]. Journal of Biotechnology, 2004, 110: 159-170
[15]	Tang Chongjian(唐崇俭), Zheng Ping(郑平), Wang Caihua(汪彩华), Zhang Jiqiang(张吉强), Chen Jianwei(陈建伟), Ding Shuang(丁爽). Granulation and characteristics of extracellular polymers of Anammox sludge in high-load EGSB reactor [J]. CIESC Journal(化工学报), 2010, 61: 732-739
[16]	Chen Y, Zhou T, Yao S, Wu J L. Ethanol production by repeated batch and continuous fermentations by Saccharomyces cerevisiae immobilized in a fibrous bed bioreactor [J]. Journal of Microbiology and Biotechnology, 2013, 23: 511-517
[17]	Dong Y, Zhang Z, Jin Y, Lu J, Cheng X, Li J, Deng Y Y, Feng Y N, Chen D. Nitrification characteristics of nitrobacteria immobilized in waterborne polyurethane in wastewater of corn-based ethanol fuel production [J]. Journal of Environmental Sciences, 2012, 24: 999-1005
[18]	Li Zebing(李泽兵), Liu Changjing(刘常敬), Zhao Baihang(赵白航), Ma Jiaxuan(马家轩), Wang Xiaoyi(王晓毅), Li Jun(李军). Activity and inhibition characteristics of Anammox and heterotrophic denitrifier bacteria in a multi-substrate system [J]. China Environmental Science(中国环境科学), 2013, 4: 648-654
[19]	Strous M, van Gerven E, Zheng P, Kuenen J G, Jetten M S. Ammonium removal from concentrated waste streams with the anaerobic ammonium oxidation (anammox) process in different reactor configurations [J]. Water Research, 1997, 31: 1955-1962
[20]	Eaton A D, Franson M A H. Standard Methods for the Examination of Water & Wastewater[M]. Washington, DC: American Public Health Association (APHA), 2005: 2001-3710
[21]	Patureau D, Bernet N, Bouchez T, Dabert P, Delgenes J, Moletta R. Biological nitrogen removal in a single aerobic reactor by association of a nitrifying ecosystem to an aerobic denitrifier [J]. Journal of Molecular Catalysis B:Enzymatic,1998, 5: 435-439
[22]	Strous M, Kuenen J G, Jetten M S. Key physiology of anaerobic ammonium oxidation [J]. Appl Environ Microb, 1999, 65:3248-3250
[23]	Jetten M S, Strous M, Pas Schoonen K T, Schalk J, Dongen U G, Graaf A A, Logemann S, Muyzer G, Loosdrecht M, Kuenen J G. The anaerobic oxidation of ammonium [J]. FEMS Microbiology Reviews, 1998, 22: 421-437
[24]	Zhang S H, Zheng P, Hua Y M. Anammox transited from denitrification in upflow biofilm reactor [J]. Journal of Environmental Sciences, 2004, 16: 1041-1045
[25]	Yu Y C, Gao D W, Tao Y. Anammox start-up in sequencing batch biofilm reactors using different inoculating sludge [J]. Applied Microbiology and Biotechnology, 2013, 97: 6057-6064
[26]	Jin Rencun(金仁村), Zheng Ping(郑平), Tang Chongjian(唐崇俭). Performance and process kinetics characteristics of ANAMMOX fixed-bed reactor [J]. Journal of Chemical Industry and Engineering (China) (化工学报), 2008, 59: 2518-2525
[27]	Gee C S, Suidan M T, Pfeffer J T. Modeling of nitrification under substrate-inhibiting conditions [J]. Journal of Environmental Engineering, 1990, 116: 18-31
[28]	Zheng Ping(郑平), Hu Baolan(胡宝兰). Kinetics of anaerobic ammonia oxidation [J]. Chinese Journal of Biotechnology(生物工程学报), 2001, 17: 193-198
[29]	Zu Bo(祖波), Zhang Daijun(张代钧), Yan Qing(阎青). Effect of trace NO2 and kinetic characteristics for anaerobic ammonium oxidation of granular sludge [J]. Environmental Science(环境科学), 2008, 29: 683-687
[30]	Tang Chongjian(唐崇俭), Xiong Lei(熊蕾), Wang Yunyan(王云燕), Zheng Ping(郑平). Kinetic characteristics of high-rate ANAMMOX granules [J]. Environmental Science(环境科学), 2013, 34: 3544-3551
[31]	Tang C J, Zheng P, Wang C H, Mahmood Q, Zhang J Q, Chen X G, Zhang L, Chen J W. Performance of high-loaded ANAMMOX UASB reactors containing granular sludge [J]. Water Research, 2011, 45: 135-144
[32]	van de Graaf A A, de Bruijn P, Robertson L A, Jetten M S, Kuenen J G. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor [J]. Microbiology,1996, 142: 2187-2196
[33]	Tang C J, Zheng P, Mahmood Q, Chen J W. Start-up and inhibition analysis of the Anammox process seeded with anaerobic granular sludge [J]. Journal of Industrial Microbiology & Biotechnology, 2009, 36: 1093-1100
[34]	Zheng Ping(郑平), Xu Xiangyang(徐向阳), Hu Baolan(胡宝兰). Novel Biological Nitrogen Removal: Theory and Technology(新型生物脱氮理论与技术)[M]. Beijing: Science Press, 2004: 76-112
[35]	Bock E, Schmidt I, Stüven R, Zart D. Nitrogen loss caused by denitrifying Nitrosomonas cells using ammonium or hydrogen as electron donors and nitrite as electron acceptor [J]. Archives of Microbiology,1995, 163: 16-20
[36]	Zhu G L, Hu Y Y, Wang Q R. Nitrogen removal performance of anaerobic ammonia oxidation co-culture immobilized in different gel carriers [J]. Water Science and Technology:A Journal of the International Association on Water Pollution Research,2009, 59: 2379-2386

包埋菌启动厌氧氨氧化反应器及其动力学性能

Performance and kinetic characteristics of immobilized granules on start-up of Anammox bioreactor

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价

[1]	金正浩, 封立杰, 李舒宏. 氨水溶液交叉型再吸收式热泵的能量及分析[J]. 化工学报, 2023, 74(S1): 53-63.
[2]	程成, 段钟弟, 孙浩然, 胡海涛, 薛鸿祥. 表面微结构对析晶沉积特性影响的格子Boltzmann模拟[J]. 化工学报, 2023, 74(S1): 74-86.
[3]	肖明堃, 杨光, 黄永华, 吴静怡. 浸没孔液氧气泡动力学数值研究[J]. 化工学报, 2023, 74(S1): 87-95.
[4]	毕丽森, 刘斌, 胡恒祥, 曾涛, 李卓睿, 宋健飞, 吴翰铭. 粗糙界面上纳米液滴蒸发模式的分子动力学研究[J]. 化工学报, 2023, 74(S1): 172-178.
[5]	于宏鑫, 邵双全. 水结晶过程的分子动力学模拟分析[J]. 化工学报, 2023, 74(S1): 250-258.
[6]	范孝雄, 郝丽芳, 范垂钢, 李松庚. LaMnO₃/生物炭催化剂低温NH₃-SCR催化脱硝性能研究[J]. 化工学报, 2023, 74(9): 3821-3830.
[7]	郑佳丽, 李志会, 赵新强, 王延吉. 离子液体催化合成2-氰基呋喃反应动力学研究[J]. 化工学报, 2023, 74(9): 3708-3715.
[8]	孟令玎, 崇汝青, 孙菲雪, 孟子晖, 刘文芳. 改性聚乙烯膜和氧化硅固定化碳酸酐酶[J]. 化工学报, 2023, 74(8): 3472-3484.
[9]	汪林正, 陆俞冰, 张睿智, 罗永浩. 基于分子动力学模拟的VOCs热氧化特性分析[J]. 化工学报, 2023, 74(8): 3242-3255.
[10]	曾如宾, 沈中杰, 梁钦锋, 许建良, 代正华, 刘海峰. 基于分子动力学模拟的Fe₂O₃纳米颗粒烧结机制研究[J]. 化工学报, 2023, 74(8): 3353-3365.
[11]	李锦潼, 邱顺, 孙文寿. 煤浆法烟气脱硫中草酸和紫外线强化煤砷浸出过程[J]. 化工学报, 2023, 74(8): 3522-3532.
[12]	杨越, 张丹, 郑巨淦, 涂茂萍, 杨庆忠. NaCl水溶液喷射闪蒸-掺混蒸发的实验研究[J]. 化工学报, 2023, 74(8): 3279-3291.
[13]	张蒙蒙, 颜冬, 沈永峰, 李文翠. 电解液类型对双离子电池阴阳离子储存行为的影响[J]. 化工学报, 2023, 74(7): 3116-3126.
[14]	史方哲, 甘云华. 超薄热管启动特性和传热性能数值模拟[J]. 化工学报, 2023, 74(7): 2814-2823.
[15]	陈雅鑫, 袁航, 刘冠章, 毛磊, 杨纯, 张瑞芳, 张光亚. 蛋白质纳米笼介导的酶自固定化研究进展[J]. 化工学报, 2023, 74(7): 2773-2782.