聚糖菌反硝化影响因素及内碳源转化特性

doi:10.11949/j.issn.0438-1157.20181076

摘要/Abstract

摘要：

在SBR反应器中以乙酸钠为碳源、 $N O_{3}^{-}$ -N为电子受体成功富集了反硝化聚糖菌，并采用批次实验进一步考察了进水C/N比（3.3,6.7,10）、电子受体（ $N O_{3}^{-}$ -N、 $N O_{2}^{-}$ -N）、碳源类型（乙酸钠、葡萄糖）对反硝化聚糖菌活性的影响及内碳源转化特性。实验结果表明，进水C/N比越高，系统 $N O_{x}^{-}$ -N去除率越高，厌氧段合成PHB越多，但进水C/N比过高会导致普通反硝化菌占优势，影响内碳源反硝化效率，进水C/N比为6.7较为合适；以 $N O_{3}^{-}$ -N为电子受体长期培养的DGAOs系统未经 $N O_{2}^{-}$ -N驯化，对 $N O_{2}^{-}$ -N同样具有良好的反硝化性能，在投加与 $N O_{3}^{-}$ -N相同浓度的 $N O_{2}^{-}$ -N后，系统 $N O_{x}^{-}$ -N去除率达89.6%；当以葡萄糖为碳源时，DPAOs在厌氧段合成的PHB的量仅为以乙酸钠为碳源时合成PHB量的79.5%，且厌氧段葡萄糖利用率仅为72.8%，远远小于乙酸钠的利用率。

关键词: 反硝化聚糖菌, 内碳源反硝化, 聚合物, 厌氧, 吸收

Abstract:

The denitrifying glycan bacteria were successfully enriched in the SBR reactor with sodium acetate as the carbon source and $N O_{3}^{-}$ -N as the electron acceptor, and the effects of the influent C/N ratio (3.3,6.7,10), electron acceptors ( $N O_{3}^{-}$ -N, $N O_{2}^{-}$ -N), carbon source types (sodium acetate, glucose) on the activity of denitrifying glycans and the conversion characteristics of internal carbon sources were further investigated by batch experiment. The results show that the higher the influent C/N ratio, the higher the $N O_{x}^{-}$ -N removal rate of the system, and the more PHB synthesis in the anaerobic section, but the influent C/N ratio is too high, which leads to the predominance of common denitrifying bacteria. The denitrification efficiency of the internal carbon source is suitable for the influent C/N ratio of 6.7. The DGAOs system with $N O_{3}^{-}$ -N as the electron acceptor for long-term cultivation is not domesticated by $N O_{2}^{-}$ -N, and has good anti- $N O_{2}^{-}$ -N. Nitrification performance, after adding the same concentration of $N O_{2}^{-}$ -N as $N O_{3}^{-}$ -N, the system NO_x^--N removal rate is 89.6%; when glucose is used as the carbon source, the amount of PHB synthesized by DPAOs in the anaerobic section is only 79.5% of PHB with sodium acetate as the carbon source, and the anaerobic glucose utilization rate is only 72.8%, which is much smaller than the utilization rate of sodium acetate.

Key words: denitrifying glycan, internal carbon source denitrification, polymers, anaerobic, absorption

中图分类号:

X 703.1

刘小芳, 郭海燕, 张胜男, 黄靓. 聚糖菌反硝化影响因素及内碳源转化特性[J]. 化工学报, 2019, 70(3): 1127-1134.

Xiaofang LIU, Haiyan GUO, Shengnan ZHANG, Liang HUANG. Influencing factors of denitrification of glycans and transformation characteristics of internal carbon sources[J]. CIESC Journal, 2019, 70(3): 1127-1134.

图/表 7

图1 SBR实验装置

Fig.1 SBR experimental device

表1 实验各阶段运行参数

Table 1 Operating parameters of each stage of the experiment

Run phase	Run time/min							Influent COD/(mg/L)	Anoxic begining $N O_{x}^{-}$ -N/(mg/L)
Run phase	Influent	Anaerobic	Anoxic	Aerobic	Settling	Effluent	Idle	Influent COD/(mg/L)	Anoxic begining $N O_{x}^{-}$ -N/(mg/L)
Ⅰ	0.5	120	—	180	40	0.5	19	150	-
Ⅱ	0.5	120	120	60	40	0.5	19	150	40
Ⅲ	0.5	120	180	—	40	0.5	19	150	40
Ⅳ	0.5	60	240	—	40	0.5	19	200	40

表1 实验各阶段运行参数

Table 1 Operating parameters of each stage of the experiment

Run phase	Run time/min							Influent COD/(mg/L)	Anoxic begining $N O_{x}^{-}$ -N/(mg/L)
Run phase	Influent	Anaerobic	Anoxic	Aerobic	Settling	Effluent	Idle	Influent COD/(mg/L)	Anoxic begining $N O_{x}^{-}$ -N/(mg/L)
Ⅰ	0.5	120	—	180	40	0.5	19	150	-
Ⅱ	0.5	120	120	60	40	0.5	19	150	40
Ⅲ	0.5	120	180	—	40	0.5	19	150	40
Ⅳ	0.5	60	240	—	40	0.5	19	200	40

图2 培养阶段COD(a)、PO43--P(b)、NO3--N及NO2--N(c)变化

Fig.2 Variations of COD(a), PO43--P(b), NO3--N and NO2--N(c) in culture stage

图3 不同进水C/N比条件下典型周期内COD(a)、NO3--N及NO2--N(b)变化

Fig.3 Variations of COD(a), NO3--N and NO2--N (b) in typical cycles under different influent C/N ratios

图4 以乙酸钠为碳源投加NO3--N(a)、NO2--N(b)及以葡糖糖为碳源投加NO3--N(c)典型周期内各指标变化

Fig.4 Addition of NO3--N(a),NO2--N(b) with sodium acetate as carbon source and change of various indexes during typical period of NO3--N(c) with glucose sugar as carbon source

图5 不同C/N比PHB(a)、糖原(b)变化情况

Fig.5 Changes of PHB(a) and glycogen(b) under different C/N ratios

图6 不同碳源条件下系统内碳源转化情况

Fig.6 Conversion of carbon sources in system under different carbon sources

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