污泥双回流-厌氧/好氧/缺氧强化内源反硝化深度脱氮

doi:10.11949/0438-1157.20221008

化工学报 ›› 2022, Vol. 73 ›› Issue (11): 5098-5105.DOI: 10.11949/0438-1157.20221008

污泥双回流-厌氧/好氧/缺氧强化内源反硝化深度脱氮

高歆婕¹(), 许载周¹, 彭永臻¹(), 黄雨薇¹, 丁静¹, 安泽铭¹, 汪传新²

^1.北京工业大学城镇污水深度处理与资源化利用国家工程实验室，北京市污水脱氮除磷处理与过程控制工程技术研究中心，北京 100124
^2.广东首汇蓝天工程科技有限公司，广东广州 510075

收稿日期:2022-07-19 修回日期:2022-08-30 出版日期:2022-11-05 发布日期:2022-12-06
通讯作者: 彭永臻
作者简介:高歆婕（1995-），女，博士研究生，907562469@qq.com
基金资助:
国家重点研发计划课题项目(2021YFC3200605);广州市产业领军人才集聚工程项目(CYLJTD-201607);高等学校学科创新引智计划（111计划）项目(基地编号D16003)

Enhance nitrogen removal via endogenous denitrification in a sludge double recirculation-anaerobic/aerobic/anoxic process

Xinjie GAO¹(), Zaizhou XU¹, Yongzhen PENG¹(), Yuwei HUANG¹, Jing DING¹, Zeming AN¹, Chuanxin WANG²

^1.National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China
^2.Guangdong Shouhui Lantian Engineering and Technology Co. Ltd. , Guangzhou 510075, Guangdong, China

Received:2022-07-19 Revised:2022-08-30 Online:2022-11-05 Published:2022-12-06
Contact: Yongzhen PENG

摘要/Abstract

摘要：

目前，如何经济有效地实现低C/N生活污水深度脱氮仍是污水处理厂面临的一个重大挑战。理论上后置反硝化可以实现深度脱氮效果，但往往由于缺乏外碳源难以同时满足经济高效脱氮。本研究建立的新型污泥双回流-厌氧/好氧/缺氧(AOA)工艺有利于富集培养以Candidatus Competibacter为主的反硝化聚糖菌(DGAOs)，能够开发与利用内碳源脱氮。该系统长期处理低C/N (3.2)实际城市生活污水，TIN去除率达91.81%，出水TIN浓度为4.36 mg·L^-1。此外，提高第二污泥回流量增加了缺氧区的MLSS同时提升了比内源反硝化速率，是深度氮去除的主要原因。随着去除效果的提升，充分的厌氧环境使得内碳源贮存量升高，脱氮效果呈现正向循环。二沉池底部产生的额外碳源随第二回流引入系统进一步强化脱氮。本研究阐明了污泥双回流-AOA系统节碳脱氮的原理与关键，为低C/N城市污水的脱氮效率提供了一种可行性。

关键词: 厌氧, 曝气, 污水, 内源反硝化, 低C/N, 强化脱氮

Abstract:

At present, how to economically and effectively realize the deep denitrification of low C/N domestic sewage is still a major challenge faced by sewage treatment plants. Theoretically, the endogenous denitrification in post anoxic zone can achieve advanced nitrogen removal. However,due to lacking external carbon source, it was often difficult to meet the discharge standard and saving-energy at the same time. In this study, a novel sludge double recirculation-anaerobic/aerobic/anoxic (AOA) process was set up. This process was conducive to the enrichment and cultivation of denitrifying glycogen accumulating organisms (DGAOs) and dominated by Candidatus Competibacte, which could develop and utilize of intracellular carbon sources. The TIN removal efficiency of 91.81% and the TIN concentration in effluent was 4.36mg·L^-1 in the long-term treatment of low C/N ratio (3.2) real municipal wastewater. In addition, the specific endogenous denitrification rate was increased with a higher MLSS in the anoxic zone after setting secondary sludge recirculation. This was the mainly reason for achieving advanced nitrogen removal. With the improvement of the nitrogen removal efficiency, the sufficient intracellular carbon sources storage in anaerobic zone was increase, and the endogenous denitrification enhanced. The nitrogen removal performance showed a positive cycle. Finally, the additional carbon source generation, which was from the bottom of the secondary sedimentation tank and introduced to the system through the secondary sludge recirculation, further enhanced the nitrogen removal. This study clarified the mechanisms and key factors for advanced nitrogen removal in sludge double recirculation-AOA system, and provided feasibility for the increased nitrogen removal efficiency in low C/N municipal wastewater treatment.

Key words: anaerobic, aeration, waste water, endogenous denitrification, low C/N ratio, enhanced nitrogen removal

中图分类号:

X 703.1

高歆婕, 许载周, 彭永臻, 黄雨薇, 丁静, 安泽铭, 汪传新. 污泥双回流-厌氧/好氧/缺氧强化内源反硝化深度脱氮[J]. 化工学报, 2022, 73(11): 5098-5105.

Xinjie GAO, Zaizhou XU, Yongzhen PENG, Yuwei HUANG, Jing DING, Zeming AN, Chuanxin WANG. Enhance nitrogen removal via endogenous denitrification in a sludge double recirculation-anaerobic/aerobic/anoxic process[J]. CIESC Journal, 2022, 73(11): 5098-5105.

图/表 7

图1 污泥双回流-AOA系统模型图

Fig.1 Schematic diagram of the AOA system

图2 污泥双回流-AOA系统长期脱氮效果

Fig.2 Long-term nitrogen removal performances in the AOA system

图3 污泥双回流-AOA系统不同区域污染浓度变化

Fig.3 The variations of pollutant concentration in different zones of AOA system

图4 污泥双回流-AOA系统内的功能微生物

Fig.4 Functional microorganisms in AOA system

图5 污泥双回流-AOA系统在不同第二污泥回流比下的TIN损失以及出水TIN浓度

Fig.5 The TIN loss and the TIN concentration in effluent in the AOA system with different R2

图6 MLSS对氮去除的影响

Fig.6 The effect of MLSS on nitrogen removal

图7 长期运行出水与二沉池底部的COD与NH4+-N浓度差值

Fig.7 The difference of COD and NH4+-N concentration between the effluent and the bottom of the secondary sedimentation tank during the long-term operation

参考文献 34

1	Gruber N, Galloway J N. An Earth-system perspective of the global nitrogen cycle[J]. Nature, 2008, 451(7176): 293-296.
2	Yu C Q, Huang X, Chen H, et al. Managing nitrogen to restore water quality in China[J]. Nature, 2019, 567(7749): 516-520.
3	Winkler M, Coats E R, Brinkman C K. Advancing post-anoxic denitrification for biological nutrient removal[J]. Water Research, 2011, 45(18): 6119-6130.
4	Zhu G B, Peng Y Z, Wang S Y, et al. Effect of influent flow rate distribution on the performance of step-feed biological nitrogen removal process[J]. Chemical Engineering Journal, 2007, 131(1/2/3): 319-328.
5	刘小芳, 郭海燕, 张胜男, 等. 聚糖菌反硝化影响因素及内碳源转化特性[J]. 化工学报, 2019, 70(3): 1127-1134.
	Liu X F, Guo H Y, Zhang S N, et al. Influencing factors of denitrification of glycans and transformation characteristics of internal carbon sources[J]. CIESC Journal, 2019, 70(3): 1127-1134.
6	Ge S J, Peng Y Z, Wang S Y, et al. Nitrite accumulation under constant temperature in anoxic denitrification process: the effects of carbon sources and COD/NO 3 - -N[J]. Bioresource Technology, 2012, 114: 137-143.
7	Gao X J, Zhang T, Wang B, et al. Advanced nitrogen removal of low C/N ratio sewage in an anaerobic/aerobic/anoxic process through enhanced post-endogenous denitrification[J]. Chemosphere, 2020, 252: 126624.
8	Zhao W H, Huang Y, Wang M X, et al. Post-endogenous denitrification and phosphorus removal in an alternating anaerobic/oxic/anoxic (AOA) system treating low carbon/nitrogen (C/N) domestic wastewater[J]. Chemical Engineering Journal, 2018, 339: 450-458.
9	Wang X X, Wang S Y, Zhao J, et al. Combining simultaneous nitrification-endogenous denitrification and phosphorus removal with post-denitrification for low carbon/nitrogen wastewater treatment[J]. Bioresource Technology, 2016, 220: 17-25.
10	王少坡, 彭永臻, 于德爽, 等. 常温短程内源反硝化生物脱氮[J]. 北京工业大学学报, 2005, 31(3): 298-302.
	Wang S P, Peng Y Z, Yu D S, et al. Biological nitrogen removal by endogenous denitrification via nitrite at normal temperature[J]. Journal of Beijing Polytechnic University, 2005, 31(3): 298-302.
11	王晓霞, 王淑莹, 赵骥, 等. 厌氧/好氧SNEDPR系统处理低C/N污水的优化运行[J]. 中国环境科学, 2016, 36(9): 2672-2680.
	Wang X X, Wang S Y, Zhao J, et al. Optimization for low C/N sewage treatment in an anaerobic/aerobic simultaneous nitrification-endogenous denitrification and phosphorous removal system[J]. China Environmental Science, 2016, 36(9): 2672-2680.
12	Miao L, Wang S Y, Li B K, et al. Effect of carbon source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate[J]. Water Research, 2016, 100: 405-412.
13	Liu J J, Yuan Y, Li B K, et al. Enhanced nitrogen and phosphorus removal from municipal wastewater in an anaerobic-aerobic-anoxic sequencing batch reactor with sludge fermentation products as carbon source[J]. Bioresource Technology, 2017, 244: 1158-1165.
14	邱圣杰, 刘瑾瑾, 李夕耀, 等. 剩余污泥碱性发酵产物对硝化过程及性能的影响[J]. 环境科学, 2020, 41(3): 1418-1424.
	Qiu S J, Liu J J, Li X Y, et al. Effect of alkaline sludge fermentation products on the nitrification process and performance[J]. Environmental Science, 2020, 41(3): 1418-1424
15	Vocks M, Adam C, Lesjean B, et al. Enhanced post-denitrification without addition of an external carbon source in membrane bioreactors[J]. Water Research, 2005, 39(14): 3360-3368.
16	王少坡, 彭永臻, 王淑莹, 等. 温度和污泥浓度对短程内源反硝化脱氮的影响[J]. 环境科学与技术, 2005, 28(4): 85-86, 103.
	Wang S P, Peng Y Z, Wang S Y, et al. Effects of temperature and MLSS on endogenous denitrification via nitrite[J]. Environmental Science and Technology, 2005, 28(4): 85-86, 103.
17	Gilcreas F W. Standard methods for the examination of water and waste water[J]. American Journal of Public Health and the Nation’s Health, 1966, 56(3): 387-388.
18	Oehmen A, Zeng R J, Yuan Z G, et al. Anaerobic metabolism of propionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems[J]. Biotechnology and Bioengineering, 2005, 91(1): 43-53.
19	Zeng R J, van Loosdrecht M C M, Yuan Z G, et al. Metabolic model for glycogen-accumulating organisms in anaerobic/aerobic activated sludge systems[J]. Biotechnology and Bioengineering, 2003, 81(1): 92-105.
20	McIlroy S J, Albertsen M, Andresen E K, et al. ‘Candidatus Competibacter’-lineage genomes retrieved from metagenomes reveal functional metabolic diversity[J]. The ISME Journal, 2014, 8(3): 613-624.
21	Gao X J, Xu Z Z, Peng Y Z, et al. The nitrification recovery capacity is the key to enhancing nitrogen removal in the AOA system at low temperatures[J]. Science of the Total Environment, 2022, 818: 151674.
22	Ji J T, Peng Y Z, Li X Y, et al. Stable long-term operation and high nitrite accumulation of an endogenous partial-denitrification (EPD) granular sludge system under mainstream conditions at low temperature[J]. Bioresource Technology, 2019, 289: 121634.
23	Ji J T, Peng Y Z, Wang B, et al. Achievement of high nitrite accumulation via endogenous partial denitrification (EPD)[J]. Bioresource Technology, 2017, 224: 140-146.
24	Chen L P, Chen H, Hu Z K, et al. Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems[J]. Water Research, 2022, 216: 118258.
25	Liu R B, Hao X D, Chen Q, et al. Research advances of Tetrasphaera in enhanced biological phosphorus removal: a review[J]. Water Research, 2019, 166: 115003.
26	Oehmen A, Lemos P C, Carvalho G, et al. Advances in enhanced biological phosphorus removal: from micro to macro scale[J]. Water Research, 2007, 41(11): 2271-2300.
27	Ginige M P, Keller J, Blackall L L. Investigation of an acetate-fed denitrifying microbial community by stable isotope probing, full-cycle rRNA analysis, and fluorescent in situ hybridization-microautoradiography[J]. Applied and Environmental Microbiology, 2005, 71(12): 8683-8691.
28	Third K A, Gibbs B, Newland M, et al. Long-term aeration management for improved N-removal via SND in a sequencing batch reactor[J]. Water Research, 2005, 39(15): 3523-3530.
29	Wang X F, Oehmen A, Freitas E B, et al. The link of feast-phase dissolved oxygen (DO) with substrate competition and microbial selection in PHA production[J]. Water Research, 2017, 112: 269-278.
30	Zhou Z, Shen X L, Jiang L M, et al. Modeling of multimode anaerobic/anoxic/aerobic wastewater treatment process at low temperature for process optimization[J]. Chemical Engineering Journal, 2015, 281: 644-650.
31	Ge S J, Peng Y Z, Wang S Y, et al. Enhanced nutrient removal in a modified step feed process treating municipal wastewater with different inflow distribution ratios and nutrient ratios[J]. Bioresource Technology, 2010, 101(23): 9012-9019.
32	彭永臻, 马斌. 低C/N比条件下高效生物脱氮策略分析[J]. 环境科学学报, 2009, 29(2): 225-230.
	Peng Y Z, Ma B. Review of biological nitrogen removal enhancement technologies and processes under low C/N ratio[J]. Acta Scientiae Circumstantiae, 2009, 29(2): 225-230.
33	Wang Y Y, Lin X M, Zhou D, et al. Nitric oxide and nitrous oxide emissions from a full-scale activated sludge anaerobic/anoxic/oxic process[J]. Chemical Engineering Journal, 2016, 289: 330-340.
34	Li J W, Peng Y Z, Zhang L, et al. Quantify the contribution of anammox for enhanced nitrogen removal through metagenomic analysis and mass balance in an anoxic moving bed biofilm reactor[J]. Water Research, 2019, 160: 178-187.

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污泥双回流-厌氧/好氧/缺氧强化内源反硝化深度脱氮

Enhance nitrogen removal via endogenous denitrification in a sludge double recirculation-anaerobic/aerobic/anoxic process

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