化工学报 ›› 2021, Vol. 72 ›› Issue (11): 5810-5819.DOI: 10.11949/0438-1157.20210634
收稿日期:
2021-05-11
修回日期:
2021-07-08
出版日期:
2021-11-05
发布日期:
2021-11-12
通讯作者:
张肖静
作者简介:
张肖静(1986—),女,副教授,基金资助:
Xiaojing ZHANG(),Bingbing MA,Han ZHANG,Denghui WEI,Hongli ZHANG,Hao HU,Zirui ZHAO
Received:
2021-05-11
Revised:
2021-07-08
Online:
2021-11-05
Published:
2021-11-12
Contact:
Xiaojing ZHANG
摘要:
抗生素在世界范围内使用广泛,目前已经在多种废水中检测到了不同种类的抗生素。以磺胺甲唑(SMX)和土霉素(OTC)为代表,研究其对厌氧氨氧化过程的短期(6 h)和长期(22 d)影响。结果表明:在实验浓度中(1、10、100、1000 μg/L),OTC的短期抑制阈值为10 μg/L,将厌氧氨氧化活性由14.6降至12.0 mg/(h·g SS);长期抑制阈值为1000 μg/L,将厌氧氨氧化活性降至11.1 mg/(h·g SS);1~1000 μg/L的SMX对厌氧氨氧化生物膜无显著影响。厌氧氨氧化生物膜对SMX的响应比对OTC的响应更加快速,可以分泌大量的胞外聚合物以抵抗SMX,同时可以诱导反硝化菌降解SMX。OTC导致厌氧氨氧化生物膜的微生物多样性增加,而SMX则促使优势微生物的丰度增加,同时诱导反硝化菌的相对丰度从0.01%最高增加至14.9%,有助于抵抗SMX的抑制。因此,厌氧氨氧化可以处理含有微量SMX的废水,在处理含OTC的废水时,则需要经过长期驯化。
中图分类号:
张肖静,马冰冰,张涵,位登辉,张红丽,胡浩,赵子睿. 厌氧氨氧化工艺处理不同抗生素废水的性能比较[J]. 化工学报, 2021, 72(11): 5810-5819.
Xiaojing ZHANG,Bingbing MA,Han ZHANG,Denghui WEI,Hongli ZHANG,Hao HU,Zirui ZHAO. Comparison of the performance of Anammox process in the treatment of wastewater from different antibiotics[J]. CIESC Journal, 2021, 72(11): 5810-5819.
样品 | 序列数 | OTU数 | Shannon指数 | ACE指数 | Chao1指数 | Simpson指数 | Candidatus Kuenenia | Denitratisoma |
---|---|---|---|---|---|---|---|---|
OTC0 | 55534 | 581 | 3.55 | 943.4 | 796.1 | 0.077 | 16.44 | 0.14 |
OTC1 | 72598 | 695 | 3.89 | 1061.8 | 949.4 | 0.074 | 23.43 | 0.1 |
OTC10 | 65062 | 665 | 4.26 | 813.3 | 794.7 | 0.035 | 10.69 | 0.37 |
OTC100 | 76460 | 724 | 3.43 | 943.9 | 915.3 | 0.161 | 37.93 | 1.19 |
OTC1000 | 86537 | 787 | 4.23 | 948.3 | 935.5 | 0.049 | 17.82 | 4.16 |
SMX0 | 82848 | 594 | 3.36 | 729.7 | 737.0 | 0.110 | 13.85 | 0.01 |
SMX1 | 73244 | 698 | 3.83 | 921.1 | 868.5 | 0.066 | 9.43 | 13.89 |
SMX10 | 75556 | 663 | 3.76 | 883.2 | 808.9 | 0.061 | 20.19 | 3.29 |
SMX100 | 64538 | 617 | 3.92 | 832.6 | 904.1 | 0.050 | 14.31 | 14.94 |
SMX1000 | 73652 | 661 | 3.95 | 892.2 | 936.0 | 0.048 | 14.15 | 10.43 |
表1 不同生物膜样品的多样性指数和主要功能微生物的相对丰度
Table 1 The diversity index of different biofilm samples and the relative abundance of main functional microorganisms
样品 | 序列数 | OTU数 | Shannon指数 | ACE指数 | Chao1指数 | Simpson指数 | Candidatus Kuenenia | Denitratisoma |
---|---|---|---|---|---|---|---|---|
OTC0 | 55534 | 581 | 3.55 | 943.4 | 796.1 | 0.077 | 16.44 | 0.14 |
OTC1 | 72598 | 695 | 3.89 | 1061.8 | 949.4 | 0.074 | 23.43 | 0.1 |
OTC10 | 65062 | 665 | 4.26 | 813.3 | 794.7 | 0.035 | 10.69 | 0.37 |
OTC100 | 76460 | 724 | 3.43 | 943.9 | 915.3 | 0.161 | 37.93 | 1.19 |
OTC1000 | 86537 | 787 | 4.23 | 948.3 | 935.5 | 0.049 | 17.82 | 4.16 |
SMX0 | 82848 | 594 | 3.36 | 729.7 | 737.0 | 0.110 | 13.85 | 0.01 |
SMX1 | 73244 | 698 | 3.83 | 921.1 | 868.5 | 0.066 | 9.43 | 13.89 |
SMX10 | 75556 | 663 | 3.76 | 883.2 | 808.9 | 0.061 | 20.19 | 3.29 |
SMX100 | 64538 | 617 | 3.92 | 832.6 | 904.1 | 0.050 | 14.31 | 14.94 |
SMX1000 | 73652 | 661 | 3.95 | 892.2 | 936.0 | 0.048 | 14.15 | 10.43 |
Phylum | Class | Order | Family | Genus | 相对丰度/ % | 序号 | 测序OTU 编号 |
---|---|---|---|---|---|---|---|
Planctomycetes | Brocadiae | Brocadiales | Brocadiaceae | Candidatus Kuenenia | 17.98 | OTU1 | Otu2 |
Proteobacteria | Gammaproteobacteria | Betaproteobacteriales | Rhodocyclaceae | Denitratisoma | 4.57 | OTU2 | Otu5 |
Gammaproteobacteria | Betaproteobacteriales | Nitrosomonadaceae | Nitrosomonas | 4.97 | OTU3 | Otu11 | |
Gammaproteobacteria | Betaproteobacteriales | Burkholderiaceae | Acidovorax | 2.37 | OTU4 | Otu13 | |
Gammaproteobacteria | Betaproteobacteriales | Burkholderiaceae | Ellin6067 | 2.58 | OTU7 | Otu353 | |
Gammaproteobacteria | Betaproteobacteriales | Burkholderiaceae | Limnobacter | 2.4 | OTU8 | Otu354 | |
Alphaproteobacteria | Rhizobiales | Xanthobacteraceae | unclassified Xanthobacteraceae | 3.41 | OTU9 | Otu10529 | |
Patescibacteria | Saccharimonadia | Saccharimonadales | unclassified Saccharimonadales | unclassified Saccharimonadales | 5.21 | OTU12 | Otu10304 |
Acidobacteria | Holophagae | Subgroup_7 | unclassified Subgroup_7 | unclassified Subgroup_7 | 1.69 | OTU14 | Otu4 |
Armatimonadetes | Fimbriimonadia | Fimbriimonadales | Fimbriimonadaceae | unclassified Fimbriimonadaceae | 1.81 | OTU15 | Otu10530 |
Deinococcus- Thermus | Deinococci | Deinococcales | Trueperaceae | Truepera | 2.43 | OTU16 | Otu11498 |
unclassified Bacteria | Acidimicrobiia | Actinomarinales | unclassified Actinomarinales | unclassified Actinomarinales | 2.12 | OTU17 | Otu10305 |
表2 优势OTU(>1%)的生物学分类及相对丰度
Table 2 The taxonomic results and relative abundances of the dominated OTU (>1%)
Phylum | Class | Order | Family | Genus | 相对丰度/ % | 序号 | 测序OTU 编号 |
---|---|---|---|---|---|---|---|
Planctomycetes | Brocadiae | Brocadiales | Brocadiaceae | Candidatus Kuenenia | 17.98 | OTU1 | Otu2 |
Proteobacteria | Gammaproteobacteria | Betaproteobacteriales | Rhodocyclaceae | Denitratisoma | 4.57 | OTU2 | Otu5 |
Gammaproteobacteria | Betaproteobacteriales | Nitrosomonadaceae | Nitrosomonas | 4.97 | OTU3 | Otu11 | |
Gammaproteobacteria | Betaproteobacteriales | Burkholderiaceae | Acidovorax | 2.37 | OTU4 | Otu13 | |
Gammaproteobacteria | Betaproteobacteriales | Burkholderiaceae | Ellin6067 | 2.58 | OTU7 | Otu353 | |
Gammaproteobacteria | Betaproteobacteriales | Burkholderiaceae | Limnobacter | 2.4 | OTU8 | Otu354 | |
Alphaproteobacteria | Rhizobiales | Xanthobacteraceae | unclassified Xanthobacteraceae | 3.41 | OTU9 | Otu10529 | |
Patescibacteria | Saccharimonadia | Saccharimonadales | unclassified Saccharimonadales | unclassified Saccharimonadales | 5.21 | OTU12 | Otu10304 |
Acidobacteria | Holophagae | Subgroup_7 | unclassified Subgroup_7 | unclassified Subgroup_7 | 1.69 | OTU14 | Otu4 |
Armatimonadetes | Fimbriimonadia | Fimbriimonadales | Fimbriimonadaceae | unclassified Fimbriimonadaceae | 1.81 | OTU15 | Otu10530 |
Deinococcus- Thermus | Deinococci | Deinococcales | Trueperaceae | Truepera | 2.43 | OTU16 | Otu11498 |
unclassified Bacteria | Acidimicrobiia | Actinomarinales | unclassified Actinomarinales | unclassified Actinomarinales | 2.12 | OTU17 | Otu10305 |
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