玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较

doi:10.11949/j.issn.0438-1157.20151570

化工学报 ›› 2016, Vol. 67 ›› Issue (5): 2084-2092.DOI: 10.11949/j.issn.0438-1157.20151570

玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较

田顺风, 程力, 顾正彪, 洪雁, 李兆丰, 李才明

江南大学食品科学与技术国家重点实验室, 江苏无锡 214122;
江南大学食品学院, 江苏无锡 214122;
江南大学食品营养与安全协同创新中心, 江苏无锡 214122

收稿日期:2015-10-19 修回日期:2016-01-08 出版日期:2016-05-05 发布日期:2016-05-05
通讯作者: 程力
基金资助:
国家自然科学基金重点项目(31230057);国家自然科学基金项目(31371787);十二五科技部国家重点科技研究与发展规划项目(2012BAD34B07)。

Comparison of different lignin removal processes for corn stover on cellulase adsorption and enzymatic hydrolysis

TIAN Shunfeng, CHENG Li, GU Zhengbiao, HONG Yan, LI Zhaofeng, LI Caiming

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China;
School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China;
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu, China

Received:2015-10-19 Revised:2016-01-08 Online:2016-05-05 Published:2016-05-05
Supported by:
supported by the Key Program of the National Natural Science Foundation of China (31230057), the National Natural Science Foundation of China (31371787) and the Twelfth Five-Year National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2012BAD34B07).

摘要/Abstract

摘要：

利用不同预处理方法获得的玉米秸秆底物研究木质素脱除对纤维素酶吸附量及酶解效率的影响。相比于其他处理方法,2%(质量分数)NaOH处理的底物具有最高的木质素脱除率(85%),最高的底物可及性[4.7 mg·(g 葡聚糖)^-1]及酶解效率(18.9%)。通过对不同处理获得的底物进行Langmuir吸附等温曲线模拟,获得了最大吸附量(W_max)与吸附平衡常数(K),且木质纤维素酶水解效率与纤维素酶吸附量具有很好的线性关系(R²>0.8),表明脱除木质素能很好地提高底物可及性与酶解效率。然而,提高NaOH浓度(3%,4%)进一步脱除木质素时,底物可及性与碳水化合物转化为单糖的效率反而明显下降。因此,适当脱除木质素而提高底物对纤维素酶的可及性将有助于获得更有效的酶水解效果。

关键词: 玉米秸秆, 生物质, 生物能源, 纤维素酶吸附, 预处理, 木质素脱除, 酶水解效率

Abstract:

Experiments were conducted for various pretreated substrates to investigate the impact of lignin content on cellulase adsorption and substrate digestibility. Compared with other treatments, 2% (mass) NaOH pretreated solids with the highest level of lignin removal (85%) exhibited the highest accessibility to cellulase [4.7 mg protein·(g glucan)^-1] and enzymatic digestibility (18.9%). The obtained maximum adsorption capacity (W_max) and equilibrium constant (K) derived from fitting the Langmuir adsorption isotherm for different delignified substrates indicated that the removal of lignin benefited cellulase adsorption. The relationship between cellulase adsorption capacities and enzymatic digestibility for raw and pretreated solids correlated well (R²>0.8), supporting the hypothesis that carbohydrate conversion was primarily dominated by enhancing substrate accessibility owing to lignin removal. Nevertheless, further delignification by NaOH with concentrations of 3% (mass) and 4% (mass) was unfavorable to improving substrate accessibility to cellulase and enhancing carbohydrates conversion to monosaccharide. It appeared that the appropriate delignification to some degree was a significant pretreatment factor to be taken into consideration to achieve more effectively enzymatic digestibility.

Key words: corn stover, biomass, bioenergy, cellulase adsorption, pretreatment, lignin removal, enzymatic digestibility

中图分类号:

TS721.1

田顺风, 程力, 顾正彪, 洪雁, 李兆丰, 李才明. 玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较[J]. 化工学报, 2016, 67(5): 2084-2092.

TIAN Shunfeng, CHENG Li, GU Zhengbiao, HONG Yan, LI Zhaofeng, LI Caiming. Comparison of different lignin removal processes for corn stover on cellulase adsorption and enzymatic hydrolysis[J]. CIESC Journal, 2016, 67(5): 2084-2092.

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玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较

Comparison of different lignin removal processes for corn stover on cellulase adsorption and enzymatic hydrolysis

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