游离氨抑制Nitrobacter活性动力学试验

doi:10.11949/j.issn.0438-1157.20180569

化工学报 ›› 2018, Vol. 69 ›› Issue (10): 4386-4393.DOI: 10.11949/j.issn.0438-1157.20180569

游离氨抑制Nitrobacter活性动力学试验

孙洪伟^1,2, 于雪^1,2, 李维维³, 祁国平³, 马娟^1,2, 吕心涛⁴, 吕慧⁵

1. 兰州交通大学环境与市政工程学院, 甘肃兰州 730070;
2. 甘肃省污水处理行业技术中心, 甘肃兰州 730070;
3. 甘肃省轻工研究院, 甘肃兰州 730070;
4. 北京城市排水集团有限责任公司科技研发中心, 北京 100032;
5. 中山大学环境科学与工程学院, 广东广州 510275

收稿日期:2018-05-28 修回日期:2018-07-09 出版日期:2018-10-05 发布日期:2018-10-05
通讯作者: 吕慧
基金资助:
国家自然科学基金项目（51668031）；兰州交通大学“百名青年优秀人才培养计划”项目（152022）；甘肃省重点研发计划-工业类项目（17YF1GA009）；深圳市科技计划项目（JSGG20170824101458515）。

Inhibitory kinetics of free ammonia on Nitrobacter

SUN Hongwei^1,2, YU Xue^1,2, LI Weiwei³, QI Guoping³, MA Juan^1,2, LÜ Xintao⁴, LÜ Hui⁵

1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China;
2. Gansu Sewage Treatment Industry Technical Center, Lanzhou 730070, Gansu, China;
3. Gansu Province Light Industry Research Institute, Lanzhou 730070, Gansu, China;
4. Research and Development Center of Beijing Drainage Group Technology, Beijing 100032, China;
5. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, Guangdong, China

Received:2018-05-28 Revised:2018-07-09 Online:2018-10-05 Published:2018-10-05
Supported by:
supported by the National Natural Science Foundation of China (51668031), the Lanzhou Jiaotong University "100 Young People Training Plan" Project(152022), the Gansu Province Key Research and Development Programs-Industrial Projects(17YF1GA009) and the Shenzhen Science and Technology Plan Projects (JSGG20170824101458515).

摘要/Abstract

摘要：

为探究游离氨（FA）对亚硝酸盐氧化细菌中硝化杆菌属（Nitrobacter）活性抑制动力学影响，本试验采用序批式活性污泥（SBR）反应器，在通过改变系统进水FNA浓度达到富集Nitrobacter基础上，以富含Nitrobacter污泥为对象（宏基因组物种注释和丰度分析显示Nitrobacter占细菌总数40.3%），基于批次试验，考察不同FA浓度梯度下亚硝酸盐氧化过程Nitrobacter比耗氧速率（SOUR）变化规律，进而采用非基质抑制动力学模型对试验数据进行拟合。结果表明，当FA>7.3 mg·L^-1时，SOUR随着FA浓度的升高而降低。尤其当FA浓度高于22.2 mg·L^-1时，SOUR降为0 g N·（g VSS·d）^-1，最大比耗氧速率为0.62 g N·（g VSS·d）^-1。此外，基于Nitrobacter活性抑制程度判断，FA抑制Nitrobacter活性抑制类型符合非竞争性可逆抑制动力学。

关键词: 游离氨, 硝化杆菌, 比耗氧速率, 动力学, 宏基因组, 生物反应器, 需氧

Abstract:

A sequencing batch reactor (SBR) was operated in this study to investigate the inhibitory kinetics of free ammonia (FA) on Nitrobacter. At the beginning of the experiment, FNA concentration in influent was changed to enrich Nitrobacter. Meanwhile, metagenomic species annotation and abundance analysis showed that Nitrobacter accounted for 40.3% of the total bacterial population. Then, the sludge enriched of Nitrobacter was used to study the variation rule of the specific oxygen uptake rate (SOUR) during nitrite oxidation process of batch tests. Furthermore, non-substrate inhibition model of FA inhibition on Nitrobacter activity was fitted. The results showed that the SOUR decreased with the increase of FA concentration as FA>7.3 mg·L^-1. In particular, the SOUR was maintained at 0 g N·(g VSS·d)^-1 when FA concentration was higher than 22.2 mg·L^-1. Moreover, maximum specific oxygen uptake rate was 0.62 g N·(g VSS·d)^-1. In addition, based on the degree of inhibition of Nitrobacter activity, the inhibition type of FA inhibition on Nitrobacter activity is in accordance with non-competitive reversible inhibition kinetics.

Key words: free ammonia, Nitrobacter, specific oxygen uptake rate, kinetic, metagenome, bioreactors, aerobic

中图分类号:

TQ028.8

孙洪伟, 于雪, 李维维, 祁国平, 马娟, 吕心涛, 吕慧. 游离氨抑制Nitrobacter活性动力学试验[J]. 化工学报, 2018, 69(10): 4386-4393.

SUN Hongwei, YU Xue, LI Weiwei, QI Guoping, MA Juan, LÜ Xintao, LÜ Hui. Inhibitory kinetics of free ammonia on Nitrobacter[J]. CIESC Journal, 2018, 69(10): 4386-4393.

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游离氨抑制Nitrobacter活性动力学试验

Inhibitory kinetics of free ammonia on Nitrobacter

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