基于约氏不动杆菌的萘生物降解特性

doi:10.11949/j.issn.0438-1157.20151992

化工学报 ›› 2016, Vol. 67 ›› Issue (9): 3981-3987.DOI: 10.11949/j.issn.0438-1157.20151992

基于约氏不动杆菌的萘生物降解特性

姜岩, 张晓华, 杨颖, 张贤明

重庆工商大学废油资源化技术与装备教育部工程研究中心, 重庆 400067

收稿日期:2016-01-03 修回日期:2016-05-06 出版日期:2016-09-05 发布日期:2016-09-05
通讯作者: 姜岩
基金资助:
国家自然科学基金项目（21376285）；重庆市自然科学基金项目（CSTC2013jcyjA20014）；重庆市应用技术开发重点项目（cstc2014yykfB90002）；重庆市教委科技资助重点项目（KJZH14210）；教育部平台科技资助项目（FYKF201506）。

Naphthalene biodegradation by Acinetobacter johnsonii

JIANG Yan, ZHANG Xiaohua, YANG Ying, ZHANG Xianming

Engineering Research Center for Waste Oil Recovery Technology and Equipment of Ministry of Education, Chongqing Technology and Business University, Chongqing 400067, China

Received:2016-01-03 Revised:2016-05-06 Online:2016-09-05 Published:2016-09-05
Supported by:
supported by the National Natural Science Foundation of China (21376285), the Natural Science Foundation of Chongqing (CSTC2013jcyjA20014), Chongqing Key Project of Applied Technology Development (cstc2014yykfB90002), Chongqing Board of Education Key Project of Science and Technology (KJZH14210) and the Scientific Platform Project, Ministry of Education (FYKF201506).

摘要/Abstract

摘要：

萘是典型的多环芳烃，毒害大、难降解，既是重要的环境污染物，又是代表性的石油烃成分，成为各类油污染场地生物修复的难点。碍于其水溶性差而制约了相关研究。利用一株从废油中分离得到的兼具石油烃降解能力和润湿反转无机杂质能力的约氏不动杆菌开展了萘的生物降解特性研究。通过研究降解因素优化出基础的降解条件；在此基础上，在50～2000 mg·L^-1范围内研究了该菌对萘的生物降解特性；并利用Monod模型和Haldane模型对比研究了该菌降解萘的动力学行为。结果表明，该菌在以2000 mg·L^-1萘为唯一碳源的特定条件下，以5%的接种量可以在146 h左右实现萘的全降解；Haldane模型适合于描述菌株的生长和底物降解行为，而Monod模型只适合于描述低浓度下萘的生物降解特性。

关键词: 萘, 降解, 石油, 环境, 约氏不动杆菌, 动力学, 底物抑制

Abstract:

Naphthalene, typical of polycyclic aromatic hydrocarbons, is highly poisonous and hardly degradable. As the typical petroleum hydrocarbon composition, it is a severe environmental pollutant, and therefore there exists great difficulty in the bioremediation of oil-contaminated sites. Due to its poor water solubility, researches on naphthalene degradation are hindered seriously. In this paper, the strain of Acinetobacter johnsonii isolated from waste oil is employed to study naphthalene biodegradation with the ability both to degrade petroleum hydrocarbon and to wet inorganic impurities. The reaction conditions are optimized based on the researches on degradation factors and the biodegradation characteristics of naphthalene are manifested in the range of 50 to 2000 mg·L^-1. The dynamics behavior of A. johnsonii is studied on the degradation of naphthalene through the comparison of the Monod model and the Haldane model. The results indicated that the strain could utilize naphthalene as sole carbon and energy source to metabolize and 2000 mg·L^-1 naphthalene could be entirely degraded within about 146 h at 5% inoculum volume and 37℃. The Haldane model is suitable to describe cell growth and the substrate degradation behaviors while the Monod model is appropriate to depict naphthalene biodegradation with low initial concentration.

Key words: naphthalene, degradation, petroleum, environment, Acinetobacter johnsonii, kinetics, substrate inhibition

中图分类号:

X592
X172

姜岩, 张晓华, 杨颖, 张贤明. 基于约氏不动杆菌的萘生物降解特性[J]. 化工学报, 2016, 67(9): 3981-3987.

JIANG Yan, ZHANG Xiaohua, YANG Ying, ZHANG Xianming. Naphthalene biodegradation by Acinetobacter johnsonii[J]. CIESC Journal, 2016, 67(9): 3981-3987.

参考文献 24

[1]	齐义彬, 李红, 曹美娜, 等. 一株多环芳烃降解菌吉2及其降解能力[J]. 化工学报, 2015, 66(3):1072-1079. QI Y B, LI H, CAO M N, et al. A PAH-degrading strain JI 2 and its biodegradation potential ability[J]. CIESC Journal, 2015, 66(3):1072-1079.
[2]	BALACHANDRAN C, DURAIPANDIYAN V, BALAKRISHNA K, et al. Petroleum and polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in Streptomyces sp. (ERI-CPDA-1) isolated from oil contaminated soil[J]. Bioresource Technology, 2012, 112(5):83-90.
[3]	FERRADJI F Z, MNIF S, BADIS A, et al. Naphthalene and crude oil degradation by biosurfactant producing Streptomyces spp. isolated from Mitidja plain soil (North of Algeria)[J]. International Biodeterioration & Biodegradation, 2014, 86(1):300-308.
[4]	宋立超, 刘灵芝, 李培军, 等. 盐碱土壤多环芳烃降解菌群筛选及其降解特性[J]. 石油学报(石油加工), 2012, 28(1):161-166. SONG L C, LIU L Z, LI P J, et al. Screening and biodegradation characteristics of polycyclic aromatic hydrocarbons-degrading consortium from saline-alkali soil[J]. Acta Petrolei Sinica (Petroleum Processing Section), 2012, 28(1):161-166.
[5]	HARITASH A K, KAUSHIK C P. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs):a review[J]. Journal of Hazardous Materials, 2009, 169(1/2/3):1-15.
[6]	李晓斌, 孙寓姣, 王红旗, 等. 焦化厂污染土壤中多环芳烃降解菌群解析[J]. 化工学报, 2010, 61(2):477-483. LI X B, SUN Y J, WANG H Q, et al. Analysis of PAH-degrading bacteria from contaminated soil at a coking plant[J]. CIESC Journal, 2010, 61(2):477-483.
[7]	高配科, 王燕森, 张宏祚, 等. 两株嗜热解烃菌对原油的降黏机制[J]. 化工学报, 2013, 64(11):4240-4245. GAO P K, WANG Y S, ZHANG H Z, et al. Mechanism of crude oil viscosity reduction by two thermophilic hydrocarbon-degrading bacteria[J]. CIESC Journal, 2013, 64(11):4240-4245.
[8]	胡艳军, 管志超, 郑小艳. 污水污泥裂解油中多环芳烃的分析[J]. 化工学报, 2013, 64(6):2227-2231. HU Y J, GUAN Z C, ZHENG X Y. Analysis on polycyclic aromatic hydrocarbons in pyrolysis oil from municipal wastewater sewage sludge[J]. CIESC Journal, 2013, 64(6):2227-2231.
[9]	姜岩, 杨颖, 张贤明. 典型多环芳烃生物降解及转化机制的研究进展[J]. 石油学报(石油加工), 2014, 30(6):1108-1121. JIANG Y, YANG Y, ZHANG X M. Review on the biodegradation and conversion mechanisms of typical polycyclic aromatic hydrocarbons[J]. Acta Petrolei Sinica (Petroleum Processing Section), 2014, 30(6):1108-1121.
[10]	LI S S, LI X, ZHAO H B, et al. Physiological role of the novel salicylaldehyde dehydrogenase NahV in mineralization of naphthalene by Pseudomonas putida ND6[J]. Microbiological Research, 2011, 166(12):643-653.
[11]	IZMALKOVA T Y, SAZONOVA O I, NAGORNIH M O, et al. The organization of naphthalene degradation genes in Pseudomonas putida strain AK5[J]. Research in Microbiology, 2013, 164(4):244-253.
[12]	PATHAK H, KANTHARIA D, MALPANI A, et al. Naphthalene degradation by Pseudomonas sp. HOB1:in vitro studies and assessment of naphthalene degradation efficiency in simulated microcosms[J]. Journal of Hazardous Materials, 2009, 166(2/3):1466-1473.
[13]	GAO Y, YU X Z, WU S C, et al. Interactions of rice (Oryza sativa L.) and PAH-degrading bacteria (Acinetobacter sp.) on enhanced dissipation of spiked phenanthrene and pyrene in waterlogged soil[J]. Science of the Total Environment, 2006, 372(12):1-11.
[14]	SEO H, KIM J, JUNG J, et al. Complexity of cell-cell interactions between Pseudomonas sp. AS1 and Acinetobacter oleivorans DR1:metabolic commensalism, biofilm formation and quorum quenching[J]. Research in Microbiology, 2012, 163(4):173-181.
[15]	JIANG Y, QI H, ZHANG X M, et al. Inorganic impurity removal from waste oil and wash-down water by Acinetobacter johnsonii[J]. Journal of Hazardous Materials, 2012, 239-240(15):289-293.
[16]	JIANG Y, ZHANG X M, CHEN G X, et al. The pilot study for waster oil removal from oilfields by Acinetobacter johnsonii using a specialized batch bioreactor[J]. Biotechnology and Bioprocess Engineering, 2012, 17(6):1300-1305.
[17]	JIANG Y, QI H, ZHANG X M, et al. Cleansing of waste oil in oilfield by pure-culture microorganisms[J]. Energy Sources, Part A:Recovery, Utilization, and Environmental Effects, 2015, 37(23):2567-2574.
[18]	JIANG Y, WEN J P, LI H M. The biodegradation of phenol at high initial concentration by the yeast Candida tropicalis[J]. Biochemical Engineering Journal, 2005, 24(3):243-247.
[19]	JIANG Y, REN N Q, CAI X, et al. Biodegradation of phenol and 4-chlorophenol by the mutant strain CTM 2[J]. Chinese Journal of Chemical Engineering, 2008, 16(5):796-800.
[20]	王春明, 李大平, 王春莲. 微杆菌3-28对萘、菲、蒽、芘的降解[J]. 应用与环境生物学报, 2009, 15(3):361-366. WANG C M, LI D P, WANG C L. Biodegradation of naphthalene, phenanthrene, anthracene and pyrene by Microbacterium sp. 3-28[J]. Chinese Journal of Applied & Environmental Biology, 2009, 15(3):361-366.
[21]	JIANG Y, WEN J P, BAI J, et al. Biodegradation of phenol at high initial concentration by Alcaligenes faecalis[J]. Journal of Hazardous Materials, 2007, 147(1/2):672-676.
[22]	JIANG Y, WEN J P, BAI J, et al. Phenol biodegradation by the yeast Candida tropicalis in the presence of m-cresol[J]. Biochemical Engineering Journal, 2006, 29(3):227-234.
[23]	JIANG Y, WEN J P, CAIYIN Q G L, et al. Mutant AFM 2 of Alcaligenes faecalis for phenol biodegradation using He-Ne laser irradiation[J]. Chemosphere, 2006, 65(7):1236-1241.
[24]	JIANG Y, CAI X, WU D, et al. Biodegradation of phenol and m-cresol by mutated Candida tropicalis[J]. Journal of Environmental Sciences, 2010, 22(4):621-626.

基于约氏不动杆菌的萘生物降解特性

Naphthalene biodegradation by Acinetobacter johnsonii

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