固定化酶催化制备乙酸正丁酯及动力学

doi:10.11949/j.issn.0438-1157.20170881

化工学报 ›› 2017, Vol. 68 ›› Issue (12): 4685-4690.DOI: 10.11949/j.issn.0438-1157.20170881

固定化酶催化制备乙酸正丁酯及动力学

王洪海, 李旭, 李春利, 刘文静

河北工业大学化工节能过程集成与资源利用国家地方联合工程实验室, 天津 300130

收稿日期:2017-07-10 修回日期:2017-09-26 出版日期:2017-12-05 发布日期:2017-12-05
通讯作者: 李春利
基金资助:
河北省科技计划项目（16214505D）。

Kinetics of n-butyl acetate prepared by immobilized enzyme

WANG Honghai, LI Xu, LI Chunli, LIU Wenjing

National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization, Hebei University of Technology, Tianjin 300130, China

Received:2017-07-10 Revised:2017-09-26 Online:2017-12-05 Published:2017-12-05
Supported by:
supported by the Science and Technology Research and Development Plan of Hebei Province, China (16214505D).

摘要/Abstract

摘要：

酯交换反应可用南极假丝酵母脂肪酶B（CALB）作催化剂，采用溶胶-凝胶法固定脂肪酶CALB得到的催化剂颗粒可用于乙酸乙酯和正丁醇的酯交换反应。首先探究了固定化酶的稳定性和重复使用性，然后在间歇反应釜内进行反应动力学实验，考察了转速、催化剂用量、酯醇比、温度等因素对反应的影响，确定了适宜的操作条件。在328.15～343.15 K下，将实验数据拟合得到反应的动力学方程，通过实验值与计算值的比较，验证此宏观动力学方程合理，可用于模拟计算。

关键词: 催化剂, 生物催化, 动力学, 酶, 固定化

Abstract:

Candida antarctica lipase B(CALB) is immobilized by entrapment in a hydrophobic silica xerogel. Firstly, the stability and reuse of immobilized enzymes are investigated and can be stored for at least 40 d at 25℃ without any significant loss of enzyme activity. Then, the transesterification of ethyl acetate with n-butanol in the presence of immobilized CALB is considered in a batch stirred tank reactor. The reaction kinetics is experimentally determined for different stirring rate, catalyst dosage, concentration and temperature. The suitable operating conditions are determined and a pseudo homogeneous kinetic model is established. The calculation data are in better agreement with the experimental data which shows that the kinetic equations are reasonable and suitable for simulation calculation.

Key words: catalyst, biocatalysis, kinetics, enzyme, immobilization

中图分类号:

TQ031.2
Q503

王洪海, 李旭, 李春利, 刘文静. 固定化酶催化制备乙酸正丁酯及动力学[J]. 化工学报, 2017, 68(12): 4685-4690.

WANG Honghai, LI Xu, LI Chunli, LIU Wenjing. Kinetics of n-butyl acetate prepared by immobilized enzyme[J]. CIESC Journal, 2017, 68(12): 4685-4690.

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固定化酶催化制备乙酸正丁酯及动力学

Kinetics of n-butyl acetate prepared by immobilized enzyme

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