图1 辅酶自循环的氧化还原级联体系的分类

Fig.1 Classification of redox neutralization systems

图2 TeSADH催化仲醇外消旋化

Fig.2 TeSADH-catalyzed racemization of secondary alcohols

图3 醇脱氢酶催化吡喃酮类化合物生成对映体γ-羟基-δ-内酯的氧化还原异构化反应

Fig.3 Oxidation-reduction isomerization of pyranone compounds to enantiomer γ-hydroxy-δ-lactone catalyzed by alcohol dehydrogenase

图4 立体选择性互补的两种醇脱氢酶催化手性仲醇的外消旋化

Fig.4 Racemization of chiral secondary alcohols catalyzed by two stereoselective complementary alcohol dehydrogenases

图5 L/D-α-羟基异己酸脱氢酶催化α-羟基羧酸外消旋化

Fig.5 Enzymatic racemization of α-hydroxycarboxylic acids by L/D-α-hydroxyisocaproate dehydrogenase

图6 一对选择性互补的ADHs催化(R)-1-苯基-1,2-乙二醇的立体转化RCR：NAD+依赖的(R)-ADH；SCR：NADPH依赖的(S)-ADH

Fig.6 A pair of selectively complementary ADHs catalyze the stereotransformation of (R)-1-phenyl-1,2-ethanediol RCR: NAD+-dependent (R)-ADH; SCR: NADPH-dependent (S)-ADH

图7 丙氨酸脱氢酶（AlaDH）偶联乳酸脱氢酶（LDH）制备L-丙氨酸及其衍生物

Fig.7 Preparing L-alanine and its derivatives by alanine dehydrogenase (AlaDH) coupled with lactate dehydrogenase (LDH)

图8 扁桃酸外消旋酶偶联D-扁桃酸脱氢酶（D-MDH）和L-氨基酸脱氢酶（L-AADH）合成L-苯甘氨酸及其衍生物

Fig.8 Coupling of mandelate racemase, D-mandelate dehydrogenase (D-MDH) and L-amino acid dehydrogenase (L-AADH) to synthesize L-phenylglycine and its derivatives

图9 醇脱氢酶（ADH）偶联胺脱氢酶（AmDH）催化醇生成手性胺

Fig.9 The formation of chiral amines from alcohols catalyzed by alcohol dehydrogenase (ADH) which coupled with amine dehydrogenase (AmDH)

图10 醇脱氢酶（ADH）偶联还原胺化酶（AspRedAm）催化醇生成手性胺

Fig.10 The formation of chiral amines from alcohols catalyzed by alcohol dehydrogenase (ADH) which coupled with reductive aminase (AspRedAm)

图11 醇脱氢酶（KRED）偶联亚胺还原酶（IRED）合成(1R,2S)-4

Fig.11 The synthesis of (1R,2S)-4 by KRED coupled with IRED

图12 吗啡脱氢酶（MDH）偶联吡啶核苷酸转氢酶（STH）和吗啡酮还原酶（MR）催化吗啡合成氢吗啡酮

Fig.12 Synthesis of dihydromorphine from morphine catalyzed by morphine dehydrogenase (MDH) coupled with pyridine nucleotide transhydrogenase (STH) and morphone reductase(MR)

图13 醇脱氢酶（ADH）偶联烯还原酶（ER）催化环己烯醇转化为环己酮

Fig.13 The conversion of cyclohexenol to cyclohexanone catalyzed by alcohol dehydrogenase (ADH) coupled with ene reductase (ER)

图14 醇脱氢酶（ADH）偶联人工转移氢化酶（ATHase）制备吡咯烷

Fig.14 Synthesis of pyrrolidine by alcohol dehydrogenase (ADH) coupled with artificial transfer hydrogenase (ATHase)

图15 烯还原酶（ER）偶联醛脱氢酶（ALDH）催化α, β-不饱和醛转化为饱和羧酸

Fig.15 Conversion of saturated carboxylic acids from α, β-unsaturated aldehydes catalyzed by (ER) coupled with aldehyde dehydrogenase (ALDH)

图16 将木糖还原酶（XR）与木糖醇脱氢酶（XDH）共固定于DNA支架上催化木糖生成木酮糖

Fig.16 Co-immobilization of xylose reductase (XR) and xylitol dehydrogenase (XDH) on DNA scaffolds to catalyze xylose to xylulose

图17 醇脱氢酶（ADH）偶联P450单加氧酶（P450 BM3）转化烷烃生成酮

Fig.17 The conversion from alkanes to ketones catalyzed by ADH coupled with P450 BM3

图18 醇脱氢酶（ADH）偶联P450单加氧酶催化(+)-瓦伦烯转化为(+)-诺卡酮

Fig.18 The conversion from (+)-valencene to (+)-nokadone catalyzed by ADH coupled with P450 monooxygenase

图19 5-外-羟基樟脑脱氢酶（FdeH）偶联P450单加氧酶合成2,5-二酮硼烷

Fig.19 The synthesis of 2,5-diketoborane by 5-exo-hydroxycamphor dehydrogenase (FdeH) coupled with P450 monooxygenase

图20 ADH偶联单加氧酶催化手性醇合成内酯

Fig.20 The synthesis of lactones from chiral alcohols catalyzed by ADH coupled with monooxygenase

图21 以环酮和二醇为底物经过单加氧酶和醇脱氢酶组成的级联体系合成内酯

Fig.21 Synthesis of lactones by a convergent cascade system consisting of monooxygenase and alcohol dehydrogenase using cyclic ketones and diols as substrates

图22 以双环[4.2.0]辛-7-酮和cis-1,2-环己烷二甲醇为底物合成手性内酯

Fig.22 Synthesis of chiral lactones by bicyclo [4.2.0]octan-7-ketone and cis-1,2-cyclohexanedimethanol

图23 HLADH偶联黄素依赖性单加氧酶（RebH/RebF）催化吲哚卤化

Fig.23 The halogenation of indole catalyzed by HLADH coupled with flavin-dependent monooxygenase (RebH/RebF)

图24 三酶级联催化醇的胺化

Fig.24 The amination of alcohol catalyzed by three-enzyme cascade system

图25 醇脱氢酶（ADH）偶联转氨酶（ωTA）和乳酸脱氢酶（LDH）转化仲醇生成手性胺

Fig.25 Conversion of sencondary alcohols to chiral amines by alcohol dehydrogenase (ADH) coupled with transaminase (ωTA) and lactate dehydrogenase (LDH)

图26 醇脱氢酶（ADH）偶联脱羧酶（PyDC）和乳酸脱氢酶（LDH）催化乙醇生成L-乳酸

Fig.26 The production of L-lactic acid from ethanol catalyzed by alcohol dehydrogenase (ADH) coupled decarboxylase (PyDC) and lactate dehydrogenase (LDH)