化工学报 ›› 2012, Vol. 63 ›› Issue (8): 2612-2616.DOI: 10.3969/j.issn.0438-1157.2012.08.038

• 能源和环境工程 • 上一篇    下一篇

木质素结构以及表面活性剂对木质素吸附纤维素酶的影响

姚兰1,赵建2,谢益民1,杨海涛1,曲音波2   

  1. 1湖北工业大学化学与环境工程学院,湖北 武汉430068;2山东大学生命科学学院,山东 济南250100
  • 收稿日期:2011-12-06 修回日期:2012-03-12 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 赵建,杨海涛

Effect of lignin structure and surfactant on cellulase adsorption by lignin

YAO Lan1,ZHAO Jian2,XIE Yimin1,YANG Haitao1,QU Yinbo2   

  • Received:2011-12-06 Revised:2012-03-12 Online:2012-08-05 Published:2012-08-05

关键词: 木质素, 表面活性剂, 结构, 吸附, 纤维素酶

Abstract: As substitute of petrol,bioethanol production from lignocelluloses is one of hot point of research in recent years.A main obstacle of bioethanol production in commercial scale is its cost.One of the most important methods to solve the key problem is raising yield of cellulose hydrolysis by enzyme.However,content and type of lignin in lignocellulosic substrates has a significant effect on the hydrolysis process,as lignin has double actions:as a physical barrier that decreases accessibility of cellulase to cellulose,and as an attractant that adsorbs cellulases i.e.forming non-productive binding.In this paper,structure and adsorption for cellulase of three kinds of ligin,milled wood lignin,Klasson lignin and alkaline lignin were studied,and various surfactants were screened and used for improving enzymatic hydrolysis of lignocelluloses because they have a great impact on cellulase adsorption on lignin.The results showed that there are different structure characteristics for the three kinds of lignin:the highest ability of adsorption for cellulase is Klasson lignin,and then milled wood lignin,the lowest alkaline lignin.The surfactant that can significantly increase enzymatic hydrolysis yield of lignocelluloses is PEG-8000, because it can make cellulase desorption from lignin and reactivation for hydrolysis of cellulose.

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