咪唑类离子液体在纤维素酶降解纤维素体系中的作用

doi:10.11949/j.issn.0438-1157.20141062

化工学报 ›› 2015, Vol. 66 ›› Issue (1): 121-125.DOI: 10.11949/j.issn.0438-1157.20141062

• 催化、动力学与反应器 • 上一篇下一篇

咪唑类离子液体在纤维素酶降解纤维素体系中的作用

范琳, 王少君, 李坤兰

大连工业大学轻工与化学工程学院, 辽宁大连 116034

收稿日期:2014-07-15 修回日期:2014-09-22 出版日期:2015-01-05 发布日期:2015-01-05
通讯作者: 王少君
基金资助:
国家自然科学基金项目(21106011)。

Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose

FAN Lin, WANG Shaojun, LI Kunlan

School of Light Industry & Chemical Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China

Received:2014-07-15 Revised:2014-09-22 Online:2015-01-05 Published:2015-01-05
Supported by:
supported by the National Natural Science Foundation of China (21106011).

摘要/Abstract

摘要：

以1-乙基-3-甲基咪唑磷酸二乙酯([Emim]DEP)、1-乙基-3-甲基咪唑醋酸([Emim]Ac)为研究对象, 考察两种离子液体(IL)对酶法降解纤维素过程的影响。采用超声辅助离子液体溶解纤维素, [Emim]DEP和[Emim]Ac分别使纤维素的结晶度降为53.6%和62.3%。在纤维素酶降解再生纤维素的过程中, 当IL的加入量小于2.0%时, 对酶解过程起促进作用, 其中加入量为0.5%时, 促进作用最强, 酶解率分别提高了11.4%和17.5%。在最优条件下(加入0.5%IL), 测量纤维素酶系中各个酶的酶活, 结果表明离子液体对β-葡萄糖苷酶起到了明显的促进作用, 分别使该酶酶活提高了120%和87%。离子液体降低了纤维二糖对葡聚糖外切酶的抑制作用, 提高了酶解效率。

关键词: 离子液体, 纤维素, 纤维素酶, 溶解, 降解

Abstract:

Taking 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) and 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) as research targets, the influence of the two ionic liquids (IL) on the process of enzymatic degradation of cellulose was investigated. By using ultrasound to assist ionic liquids in dissolving cellulose, [Emim]DEP and [Emim]Ac made the crystallinity of cellulose decrease to 53.6% and 62.3% respectively. In the process of enzymatic degradation of regenerated cellulose, when the dosage of IL was less than 2.0%, IL acted as promoter, and when the dosage of IL was 0.5%, the promoting effect of IL was the strongest. Enzymatic hydrolysis rate increased by 11.4% and 17.5% respectively. Under the optimal conditions (add in 0.5% IL), the enzyme activities of every enzyme in the cellulose system were measured. Ionic liquids had an obvious promoting effect on β-glucosidase, increasing enzyme activity by 120% and 87% respectively. Ionic liquids reduced inhibition of exoglucanase by cellubiose, and improved efficiency of enzymatic degradation of cellulose.

Key words: ionic liquids, cellulose, cellulase, dissolution, degradation

中图分类号:

TQ352.2

范琳, 王少君, 李坤兰. 咪唑类离子液体在纤维素酶降解纤维素体系中的作用[J]. 化工学报, 2015, 66(1): 121-125.

FAN Lin, WANG Shaojun, LI Kunlan. Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose[J]. CIESC Journal, 2015, 66(1): 121-125.

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咪唑类离子液体在纤维素酶降解纤维素体系中的作用

Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose

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