十字型微通道制备粒径均一的纤维素层析介质

doi:10.3969/j.issn.0438-1157.2013.02.036

化工学报 ›› 2013, Vol. 64 ›› Issue (2): 676-682.DOI: 10.3969/j.issn.0438-1157.2013.02.036

十字型微通道制备粒径均一的纤维素层析介质

童芳丽¹, 林东强¹, 刘川¹, 贠军贤², 姚善泾¹

1. 浙江大学化学工程与生物工程学系,化学工程联合国家重点实验室,浙江杭州 310027;
2. 浙江工业大学化学工程与材料学院,绿色化学合成技术国家重点实验室培育基地,浙江杭州 310032

收稿日期:2012-08-27 修回日期:2012-09-29 出版日期:2013-02-05 发布日期:2013-02-05
通讯作者: 林东强
作者简介:童芳丽(1988—),女,硕士研究生。
基金资助:
国家自然科学基金项目(21276228,21036005);高等学校博士学科点专项科研基金项目(201001011100);浙江省自然科学基金项目(LR12B06003)。

Preparation of cellulose-based chromatography matrix with uniform size by cross-flow microchannel chip

TONG Fangli¹, LIN Dongqiang¹, LIU Chuan¹, YUN Junxian², YAO Shanjing¹

1. State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China;
2. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, School of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, Zhejiang, China

Received:2012-08-27 Revised:2012-09-29 Online:2013-02-05 Published:2013-02-05
Supported by:
supported by the National Natural Science Foundation of China(21276228,21036005), Specialized Research Fund for the Doctoral Program of Higher Education(201001011100) and the Natural Science Foundation of Zhejiang Province(LR12B06003).

摘要/Abstract

摘要： 为了满足层析介质对粒径单分散的要求,利用十字型微通道的聚焦效应,形成粒径均一的纤维素微液滴,经固化成纤维素微球,再偶联配基制成了纤维素层析介质。以离子液体1-乙基-3-甲基咪唑甲基磷酸直接溶解微晶纤维素为水相,葵花籽油为油相,考察了微通道内微液滴的形成条件,优化了纤维素浓度、分散剂浓度、油水两相流速等因素,得到粒径约100 μm的纤维素微液滴,CV值小于0.2。微液滴固化再生,得到球形度良好的纤维素微球,湿真密度1.019 g·ml^-1,孔度94.6%,体均粒径105.5 μm;进一步偶联DEAE配基,制得了离子交换层析介质,离子交换容量为123.3 μmol·g^-1,牛血清白蛋白的饱和吸附容量Q_m达到220 mg·g^-1,有效扩散系数D_e为1.8×10^-11m²·s^-1,体现出较好的蛋白质吸附性能。

关键词: 微流芯片, 纤维素, 微球, 离子液体, 离子交换介质

Abstract: To prepare the cellulose-based chromatography matrix with uniform size, a cross-flow microchannel chip was used to control the droplet size of cellulose solution and then the microdroplets were solidified to obtain the monodisperse cellulose beads.In the present work, ionic liquid,[EMIM]MP, was used to dissolve the microcrystalline cellulose as water phase and sunflower oil was used as oil phase.The effects of cellulose concentration, surfactant addition, flow rates of oil phase and water phase were investigated.The optimized preparation condition was 2% cellulose solution as water phase, flow rate of water phase of 6 μl·min^-1 and flow rate of oil phase of 200 μl·min^-1.The microdroplets of cellulose solution thus obtained could be about 100 μm with the variation coefficient less than 0.2.After solidification and regeneration, cellulose beads were obtained with good sphericity.Wet density of beads was 1.019 g·ml^-1, porosity was 94.6%, and mean size was about 105.5 μm.The cellulose beads were coupled with DEAE ligand and used as weak anion-exchange resin.The ion exchange capacity was 123.3 μmol·g^-1.Static adsorption and adsorption kinetics were studied.The results showed that saturated adsorption capacity for bovine serum albumin could reach 220 mg·g^-1, and the effective diffusion coefficient was 1.8×10^-11 m²·s^-1, which showed potential application for protein separation.

Key words: microfluidic chip, cellulose, microsphere, ionic liquid, ion-exchange resin

中图分类号:

TQ352.7

童芳丽, 林东强, 刘川, 贠军贤, 姚善泾. 十字型微通道制备粒径均一的纤维素层析介质[J]. 化工学报, 2013, 64(2): 676-682.

TONG Fangli, LIN Dongqiang, LIU Chuan, YUN Junxian, YAO Shanjing. Preparation of cellulose-based chromatography matrix with uniform size by cross-flow microchannel chip[J]. CIESC Journal, 2013, 64(2): 676-682.

参考文献 17

[1]	Schwartz J A, Vykoukal J V, Gascoyne P R C.Droplet-based chemistry on a programmable micro-chip[J].Lab on a Chip- Miniaturisation for Chemistry and Biology, 2004, 4(1):11-17
[2]	Tumarkin E, Kumacheva E.Microfluidic generation of microgels from synthetic and natural polymers[J].Chemical Society Reviews, 2009, 38(8):2161-2168
[3]	Yeh C H, Zhao Q, Lee S J, Lin Y C.Using a T-junction microfluidic chip for monodisperse calcium alginate microparticles and encapsulation of nanoparticles[J].Sensors and Actuators, A:Physical, 2009, 151(2):231-236
[4]	Liu Chuan(刘川), Zhu Liying(朱丽英), Lin Dongqiang(林东强), Yao Shanjing(姚善泾).Preparation of chitosan-NaCS/TPP microcapsule with uniform size by microfluidic chip[J].CIESC Journal(化工学报),2012,63(1):216-222
[5]	Yang C H, Huang K S, Lin P W, Lin Y C.Using a cross-flow microfluidic chip and external crosslinking reaction for monodisperse TPP-chitosan microparticles[J].Sensors and Actuators, B:Chemical, 2007, 124(2):510-516
[6]	Shepherd R F, Conrad J C, Rhodes S K, Darren R, Marquez M, Weitz D A, Lewis J A.Microfluidic assembly of homogeneous and janus colloid-filled hydrogel granules[J].Langmuir, 2006, 22(21):8618-8622
[7]	Huang K S, Lu K, Yeh C S, Chung S R, Lin C H, Yang C H, Dong Y S.Microfluidic controlling monodisperse microdroplet for 5-fluorouracil loaded genipin-gelatin microcapsules[J].Journal of Controlled Release, 2009, 137(1):15-19
[8]	Liu L, Yang J P, Ju X J, Xie R, Yang L, Liang B, Chu L Y.Microfluidic preparation of monodisperse ethyl cellulose hollow microcapsules with non-toxic solvent[J].Journal of Colloid and Interface Science, 2009, 336(1):100-106
[9]	Yeh C H, Lin Y C.Using a cross-flow microfluidic chip for monodisperse UV-photopolymerized microparticles[J].Microfluidics and Nanofluidics, 2009, 6(2):277-283
[10]	Heinze T, Schwikal K, Barthel S.Ionic liquids as reaction medium in cellulose functionalization[J].Macromolecular Bioscience, 2005, 5(6):520-525
[11]	Lei Yinlin(雷引林), Lin Dongqiang(林东强), Yao Shanjing(姚善泾),Zhu Ziqiang(朱自强).Adsorption behavior of cellulose/ titanium dioxide composite beads in expanded bed[J].Journal of Chemical Industry and Engineering(China)(化工学报),2004,55(4):618-622
[12]	Shi F, Lin D Q, Phottraithip W, Yao S J.Preparation of cellulose-tungsten carbide composite beads with ionic liquid for expanded bed application[J].Journal of Applied Polymer Science, 2011, 119(6):3453-3461
[13]	Fukaya Y, Hayashi K, Wada M, Ohno H.Cellulose dissolution with polar ionic liquids under mild conditions:required factors for anions[J].Green Chemistry, 2007, 10(1):44-46
[14]	Raper A H.Unoriented particulate DEAE-cellulose ion exchange material:US, 3102113.1963-08-27
[15]	Lu Huili(卢慧丽), Lin Dongqiang(林东强), Yao Shanjing(姚善泾).Influences of ligand density and pore size on BSA adsorption on agarose-based DEAE-ion-exchange resins[J].CIESC Journal(化工学报), 2011, 62(11):3164-3170
[16]	Lei Y L, Lin D Q, Yao S J, Liu Z Z, Zhu Z Q.Physical and hydrodynamic properties of spherical cellulose-titanium dioxide composite matrix for expanded bed adsorption[J].Chinese Journal of Chemical Engineering, 2003, 11(2):141-145
[17]	Shi Fei(施霏).Preparation of cellulose beads with ionic liquid dissolution and its application.Hangzhou:Zhejiang University, 2011

十字型微通道制备粒径均一的纤维素层析介质

Preparation of cellulose-based chromatography matrix with uniform size by cross-flow microchannel chip

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