化工学报 ›› 2022, Vol. 73 ›› Issue (7): 3232-3239.DOI: 10.11949/0438-1157.20220184
收稿日期:
2022-02-09
修回日期:
2022-04-28
出版日期:
2022-07-05
发布日期:
2022-08-01
通讯作者:
吴文娟
作者简介:
黄丽菁(1997—),女,硕士研究生,基金资助:
Lijing HUANG(),Jijiao HUANG,Penghui LI,Zhinuo LIU,Kangjie JIANG,Wenjuan WU()
Received:
2022-02-09
Revised:
2022-04-28
Online:
2022-07-05
Published:
2022-08-01
Contact:
Wenjuan WU
摘要:
通过两步法对木质素进行了羟丙基磺甲基化改性,研究了羟丙基磺甲基木质素对纤维素酶水解的影响及其与酶的相互作用机制。采用红外光谱、核磁氢谱、表面电荷测定、接触角测定等方法对改性木质素的结构和表面特性进行了表征;采用耗散型石英晶体微天平(quartz crystal microbalance with dissipation, QCM-D)研究了改性木质素对纤维素酶非生产性吸附的影响。结果表明:与未改性木质素相比,羟丙基磺甲基化改性封闭了酚羟基,引入了亲水性的磺酸基团。羟丙基磺甲基化木质素具有较高的表面负电性和较低的疏水性,减少了其对纤维素酶的非生产性吸附,从而提高了纤维素的酶水解效率。
中图分类号:
黄丽菁, 黄继娇, 李鹏辉, 刘芷诺, 蒋康杰, 吴文娟. 木质素羟丙基磺甲基化改性及其对纤维素酶水解的影响[J]. 化工学报, 2022, 73(7): 3232-3239.
Lijing HUANG, Jijiao HUANG, Penghui LI, Zhinuo LIU, Kangjie JIANG, Wenjuan WU. Hydroxypropyl sulfomethylation modification of lignin and its effect on cellulase hydrolysis[J]. CIESC Journal, 2022, 73(7): 3232-3239.
样品 | 元素组成/% | 酚羟基含量/(mmol/g) | 表面电荷/(mmol/g) | ||
---|---|---|---|---|---|
C | O | S | |||
木质素 | 77.97±0.03 | 21.65±0.01 | 0.38±0.02 | 5.28±0.21 | -0.33±0.04 |
羟丙基磺甲基木质素 | 77.33±0.03 | 21.89±0.02 | 0.78±0.05 | 0.36±0.01 | -0.58±0.02 |
表1 木质素和羟丙基磺甲基木质素的表面特性
Table 1 Surface properties of lignin and hydroxypropyl sulfonmethyl lignin
样品 | 元素组成/% | 酚羟基含量/(mmol/g) | 表面电荷/(mmol/g) | ||
---|---|---|---|---|---|
C | O | S | |||
木质素 | 77.97±0.03 | 21.65±0.01 | 0.38±0.02 | 5.28±0.21 | -0.33±0.04 |
羟丙基磺甲基木质素 | 77.33±0.03 | 21.89±0.02 | 0.78±0.05 | 0.36±0.01 | -0.58±0.02 |
样品 | ( | R2 | 最大吸附量/(ng/cm2) | 不可逆吸附量/(ng/cm2) | 解吸量/(ng/cm2) | |
---|---|---|---|---|---|---|
木质素膜 | 57.81 | 0.064 | 0.92 | 341.1 | 311.9 | 29.2 |
羟丙基磺甲基木质素膜 | 50.59 | 0.053 | 0.95 | 298.5 | 255.4 | 43.1 |
表2 纤维素酶在木质素薄膜上的吸附参数
Table 2 Adsorption parameters of cellulase on lignin films
样品 | ( | R2 | 最大吸附量/(ng/cm2) | 不可逆吸附量/(ng/cm2) | 解吸量/(ng/cm2) | |
---|---|---|---|---|---|---|
木质素膜 | 57.81 | 0.064 | 0.92 | 341.1 | 311.9 | 29.2 |
羟丙基磺甲基木质素膜 | 50.59 | 0.053 | 0.95 | 298.5 | 255.4 | 43.1 |
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